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ZHX2 antibody [C1C3]

Anti-ZHX2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112232
Anti-ZHX2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112232
Anti-ZHX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112232
Anti-ZHX2 antibody [C1C3] used in Immunoprecipitation (IP). GTX112232
Anti-ZHX2 antibody [C1C3] used in Western Blot (WB). GTX112232

Cat No. GTX112232

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP-seq

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP-seq Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ZHX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zinc fingers and homeoboxes 2 , AFR1 , RAF

Cellular Localization

Nucleus

Background

The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZHX2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112232

GTX112232 ICC/IF Image

ZHX2 antibody [C1C3] detects ZHX2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZHX2 protein stained by ZHX2 antibody [C1C3] (GTX112232) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-ZHX2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112232

GTX112232 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZHX2(GTX112232) antibody at 1:200 dilution.

Anti-ZHX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112232

GTX112232 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using ZHX2(GTX112232) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ZHX2 antibody [C1C3] used in Immunoprecipitation (IP). GTX112232

GTX112232 IP Image

Immunoprecipitation of ZHX2 protein from MCF-7 whole cell extracts using 5 μg of ZHX2 antibody [C1C3] (GTX112232).
Western blot analysis was performed using ZHX2 antibody [C1C3] (GTX112232).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ZHX2 antibody [C1C3] used in Western Blot (WB). GTX112232

GTX112232 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZHX2 antibody [C1C3] (GTX112232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169