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ZHX2 antibody [C1C3]

Anti-ZHX2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112232
Anti-ZHX2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112232
Anti-ZHX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112232
Anti-ZHX2 antibody [C1C3] used in Immunoprecipitation (IP). GTX112232
Anti-ZHX2 antibody [C1C3] used in Western Blot (WB). GTX112232

Cat No. GTX112232

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP-seq

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP-seq Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ZHX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952653

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zinc fingers and homeoboxes 2 , AFR1 , RAF

Cellular Localization

Nucleus

Background

The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZHX2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112232

GTX112232 ICC/IF Image

ZHX2 antibody [C1C3] detects ZHX2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZHX2 protein stained by ZHX2 antibody [C1C3] (GTX112232) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-ZHX2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112232

GTX112232 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZHX2(GTX112232) antibody at 1:200 dilution.

Anti-ZHX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112232

GTX112232 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using ZHX2(GTX112232) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ZHX2 antibody [C1C3] used in Immunoprecipitation (IP). GTX112232

GTX112232 IP Image

Immunoprecipitation of ZHX2 protein from MCF-7 whole cell extracts using 5 μg of ZHX2 antibody [C1C3] (GTX112232).
Western blot analysis was performed using ZHX2 antibody [C1C3] (GTX112232).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ZHX2 antibody [C1C3] used in Western Blot (WB). GTX112232

GTX112232 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZHX2 antibody [C1C3] (GTX112232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf