GeneTex
United States (US)

ZIC2 antibody

GTX10550_20160330_WB_19011506_174.jpg

Cat No. GTX10550

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Human, Mouse, Rat, Dog, Chimpanzee
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ELISA 1:10000 - 1:50000
Not tested in other applications.

Predict Reactivity

Zebrafish, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide: FGRSEQYRQVASPRTDPYS conjugated to KLH, corresponding to amino acids 189-206 of Mouse Zic2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nuclear

Background

ZIC2 (zinc finger protein of the cerebellum 2) is a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. In mouse, knockout of Zic1 leads to aplasia of cerebellum and skeletal abnormalities. Mutation in Zic2 and Zic3 in mouse and/or human cause holoprosencephaly and heterotaxis, respectively. Holoprosencephaly is the most common structural anomaly of the human brain.

Research Area

DATA IMAGES
GTX10550_20160330_WB_19011506_174.jpg

GTX10550 WB Image

Western blot using GeneTex's Affinity Purified anti-ZIC2 antibody shows detection of a band ~55 kDa (arrowhead) corresponding to ZIC2 in lysates from (lane 1) mouse brain and (lane 2) rat brain. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-ZIC2 antibody. Signal was detected using standard techniques. The upper band in the mouse brain lysate represents Zic2.

Package List Price ($)
$ 319