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ZIC3 antibody [N3C3]

Anti-ZIC3 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX109987
Anti-ZIC3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109987
Anti-ZIC3 antibody [N3C3] used in Western Blot (WB). GTX109987

Cat No. GTX109987

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Zebrafish, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZIC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952655

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zic family member 3 , HTX , HTX1 , VACTERLX , ZNF203

Background

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs. [provided by RefSeq]

Database

DATA IMAGES

Anti-ZIC3 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX109987

GTX109987 IHC-Fr Image

ZIC3 antibody [N3C3] detects ZIC3 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: ZIC3 protein stained by ZIC3 antibody [N3C3] (GTX109987) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ZIC3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109987

GTX109987 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using ZIC3(GTX109987) antibody at 1:100 dilution.

Anti-ZIC3 antibody [N3C3] used in Western Blot (WB). GTX109987

GTX109987 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX109987 diluted at 1:500

REFERENCE

There are currently no references for ZIC3 antibody [N3C3] (GTX109987). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ZIC3 antibody [N3C3] (GTX109987). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169