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ZIP1 antibody

Anti-ZIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85135
Anti-ZIP1 antibody used in Western Blot (WB). GTX85135
Anti-ZIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85135

Cat. No. GTX85135

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all ZIP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Mouse Kidney Tissue Lysate(GTX27936) , Human Kidney Tissue Slide

Product Note

At least two isoforms of ZIP1 are known to exist; this antibody will detect both isoforms. ZIP1 antibody is predicted not to cross-react with other ZIP family proteins.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ZIP1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human ZIP1.The immunogen is located within amino acids 40 - 90 of ZIP1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10721822

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 39 member 1 , ZIP1 , ZIRTL

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane

Background

ZIP1, also known as ZIRTL (zinc-iron regulated transporter-like), is the first mammalian member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP1 expression is markedly downregulated in a number of cancerous tissues and is thought to function as a tumor suppressor gene in prostate cancer. More recent studies have shown that overexpression of ZIP1 and concomitant increased levels of intracellular zinc in PC-3 cells cause a significant inhibition of NF-kappaB, leading to down-regulation of the antiapoptotic protein Bcl-2, Bcl-XL, and the apoptosis inhibitor XIAP, reducing the malignant potential of prostate cancer cells in vitro and in vivo.

Database

Research Area

DATA IMAGES

Anti-ZIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85135

GTX85135 IHC-P Image

IHC-P analysis of human kidney tissue using GTX85135 ZIP1 antibody.
Working concentration : 2.5 μg/ml

Anti-ZIP1 antibody used in Western Blot (WB). GTX85135

GTX85135 WB Image

WB analysis of mouse kidney tissue lysate using GTX85135 ZIP1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-ZIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85135

GTX85135 IHC-P Image

IHC-P analysis of human kidney tissue using GTX85135 ZIP1 antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Rafa?o-Uli?ska A et al. Nutrients 2020; 12 (9) Imipramine Influences Body Distribution of Supplemental Zinc Which May Enhance Antidepressant Action.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399