GeneTex
United States (US)

ZKSCAN3 antibody [N1C2]

GTX106039_39953_IHC_19011506_325.jpg
GTX106039_IFA_19011506_494.jpg
GTX106039_43040_20171208_WB_19021719_938.jpg

Cat No. GTX106039

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

K562
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZKSCAN3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ33906 antibody, KIAA0426 antibody, ZF47 antibody, ZFP306 antibody, ZNF306 antibody, ZNF309 antibody, ZSCAN13 antibody, Zfp47 antibody, dJ874C20.1 antibody, ZKSCAN3 antibody, zinc finger protein with KRAB and SCAN domains 3 antibody, zinc finger protein zfp47 antibody, zinc finger and SCAN domain-containing protein 13 antibody, zinc finger protein 306 antibody, zinc finger protein 47 homolog antibody, zinc finger protein 309 antibody, zinc finger with KRAB and SCAN domains 3 antibody

Background

Acts as a transcriptional regulator. Binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3'. Associates with chromatin at the ITGB4 and VEGF promoters. Activates the transcription of genes associated with colon cancer progression.

Database

Research Area

DATA IMAGES
GTX106039_39953_IHC_19011506_325.jpg

GTX106039 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal lung, using ZKSCAN3(GTX106039) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106039_IFA_19011506_494.jpg

GTX106039 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZNF306 (GTX106039) antibody at 1:200 dilution.

GTX106039_43040_20171208_WB_19021719_938.jpg

GTX106039 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZKSCAN3 antibody [N1C2] (GTX106039) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf