GeneTex
United States (US)

ZNF133 antibody [N1C1-2]

GTX114896_40240_20150612_IHC_18121410_170.jpg
GTX114896_40240_IFA_18121410_286.jpg
GTX114896_WB_18121410_619.jpg
GTX114896_40240_20150612_IHC_2_18121410_205.jpg

Cat No. GTX114896

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

A549 , HeLa , HCT116

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZNF133. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zinc Finger Protein 133,Znf150,Phz-13,Phz-66,Znf133

Cellular Localization

Nucleus

Background

May be involved in transcriptional regulation as a repressor.

Database

DATA IMAGES
GTX114896_40240_20150612_IHC_18121410_170.jpg

GTX114896 IHC-P Image

ZNF133 antibody [N1C1-2] detects ZNF133 protein at nucleus in human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human breast cancer.
ZNF133 antibody [N1C1-2] (GTX114896) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114896_40240_IFA_18121410_286.jpg

GTX114896 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZNF133(GTX114896) antibody at 1:200 dilution.

GTX114896_WB_18121410_619.jpg

GTX114896 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116 (GTX27909)
7.5% SDS PAGE
GTX114896 diluted at 1:5000

GTX114896_40240_20150612_IHC_2_18121410_205.jpg

GTX114896 IHC-P Image

ZNF133 antibody [N1C1-2] detects ZNF133 protein at nucleus in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
ZNF133 antibody [N1C1-2] (GTX114896) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169