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ZNF174 antibody

Anti-ZNF174 antibody used in Western Blot (WB). GTX55226
Anti-ZNF174 antibody used in IHC (Paraffin sections) (IHC-P). GTX55226
Anti-ZNF174 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55226

Cat. No. GTX55226

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ZNF174 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of ZNF174. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger protein 174 , ZSCAN8

Cellular Localization

Nucleus

Background

This gene encodes a protein with three Cys2-His2-type zinc fingers in the carboxy-terminus, a putative nuclear localization signal, and an amino-terminus SCAN box which forms homodimers. This protein is believed to function as a transcriptional repressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Dec 2016]

Database

DATA IMAGES

Anti-ZNF174 antibody used in Western Blot (WB). GTX55226

GTX55226 WB Image

WB analysis of Jurkat (A), Raw264.7 (B), H9C2 (C) whole cell lysates using GTX55226 ZNF174 antibody.

Anti-ZNF174 antibody used in IHC (Paraffin sections) (IHC-P). GTX55226

GTX55226 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55226 ZNF174 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-ZNF174 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55226

GTX55226 ICC/IF Image

ICC/IF analysis of formalin-fixed Jurkat cells using GTX55226 ZNF174 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399