GeneTex
United States (US)

ZNF198 antibody

GTX105550_IHC_19011506_672.jpg
GTX105550_40023_IFA_mESC_19011506_930.jpg
GTX105550_40023_IFA_hESC_19011506_947.jpg
GTX105550_40023_WB_hESC_19011506_487.jpg

Cat No. GTX105550

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:50-1:500
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Positive Control

human ESC

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZNF198. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FIM antibody, MYM antibody, RAMP antibody, SCLL antibody, ZNF198 antibody, ZMYM2 antibody, fused in myeloproliferative disorders protein antibody, zinc finger protein 198 antibody, rearranged in an atypical myeloproliferative disorder antibody, zinc finger MYM-type protein 2 antibody, "zinc finger, MYM-type 2 antibody"

Background

The protein encoded by this gene is a zinc finger protein that may act as a transcription factor. The encoded protein may be part of a BHC histone deacetylase complex. Translocation of this gene with the fibroblast growth factor receptor-1 gene (FGFR1) results in a fusion gene, which may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
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Database

DATA IMAGES
GTX105550_IHC_19011506_672.jpg

GTX105550 IHC-P Image

ZNF198 antibody detects ZNF198 protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
ZNF198 antibody (GTX105550) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105550_40023_IFA_mESC_19011506_930.jpg

GTX105550 ICC/IF Image

Immunofluorescence analysis of methanol-fixed mouse ESC, using ZNF198(GTX105550) antibody at 1:500 dilution.

GTX105550_40023_IFA_hESC_19011506_947.jpg

GTX105550 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using ZNF198(GTX105550) antibody at 1:1000 dilution.

GTX105550_40023_WB_hESC_19011506_487.jpg

GTX105550 WB Image

Sample (20 ug of whole cell lysate)
A: human ESC
5% SDS PAGE
GTX105550 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169