GeneTex
United States (US)

ZNF217 antibody [N3C2], Internal

GTX104572_40618_WB_19011506_418.jpg
GTX104572_40618_IHC_19011506_357.jpg

Cat No. GTX104572

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG , MCF-7
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZNF217. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ZABC1 antibody, ZNF217 antibody, zinc finger protein 217 antibody

Cellular Localization

Nucleus

Background

Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.

Database

Research Area

DATA IMAGES
GTX104572_40618_WB_19011506_418.jpg

GTX104572 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
5% SDS PAGE
GTX104572 diluted at 1:1000

GTX104572_40618_IHC_19011506_357.jpg

GTX104572 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using ZNF217 antibody [N3C2], Internal(GTX104572) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf