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ZNF329 antibody

Anti-ZNF329 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122136
Anti-ZNF329 antibody used in Western Blot (WB). GTX122136
Anti-ZNF329 antibody used in IHC (Paraffin sections) (IHC-P). GTX122136

Cat. No. GTX122136

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZNF329 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

NT2D1

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZNF329. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174533

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger protein 329

Cellular Localization

Nucleus

Background

May be involved in transcriptional regulation.

Database

Research Area

DATA IMAGES

Anti-ZNF329 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122136

GTX122136 ICC/IF Image

ZNF329 antibody detects ZNF329 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF329 protein stained by ZNF329 antibody (GTX122136) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ZNF329 antibody used in Western Blot (WB). GTX122136

GTX122136 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX122136 diluted at 1:1000

Anti-ZNF329 antibody used in IHC (Paraffin sections) (IHC-P). GTX122136

GTX122136 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ZNF329(GTX122136) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169