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ZNF398 antibody [C1C3]

Anti-ZNF398 antibody [C1C3] used in Western Blot (WB). GTX107221
Anti-ZNF398 antibody [C1C3] used in Western Blot (WB). GTX107221
Anti-ZNF398 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107221
Anti-ZNF398 antibody [C1C3] used in Western Blot (WB). GTX107221

Cat. No. GTX107221

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ZNF398 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231 , mouse kidney , rat kidney

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZNF398. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038338

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger protein 398 , P51 , P71 , ZER6

Cellular Localization

Nucleus

Background

This gene encodes a member of the Kruppel family of C2H2-type zinc-finger transcription factor proteins. The encoded protein acts as a transcriptional activator. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described, but their full length sequence has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZNF398 antibody [C1C3] used in Western Blot (WB). GTX107221

GTX107221 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZNF398 antibody [C1C3] (GTX107221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 72 kDa, and 55 kDa. It is possibly due to alternative splicing.
Reference:PMID: 11779858

Anti-ZNF398 antibody [C1C3] used in Western Blot (WB). GTX107221

GTX107221 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZNF398 antibody [C1C3] (GTX107221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZNF398 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107221

GTX107221 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZNF398(GTX107221) antibody at 1:200 dilution.

Anti-ZNF398 antibody [C1C3] used in Western Blot (WB). GTX107221

GTX107221 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZNF398 antibody [C1C3] (GTX107221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 72 kDa, and 55 kDa. It is possibly due to alternative splicing.
Reference:PMID: 11779858

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169