Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ZNF501(GTX116521) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ZNF501 antibody [N2C3] detects ZNF501 protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF501 protein stained by ZNF501 antibody [N2C3] (GTX116521) diluted at 1:500.
Blue: Hoechst 33342 staining.

Sample (30 ug of whole cell lysate)
A: U87-MG
12% SDS PAGE
GTX116521 diluted at 1:1000