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ZNF536 antibody

Anti-ZNF536 antibody used in IHC (Paraffin sections) (IHC-P). GTX85225
Anti-ZNF536 antibody used in IHC (Paraffin sections) (IHC-P). GTX85225
Anti-ZNF536 antibody used in Western Blot (WB). GTX85225

Cat. No. GTX85225

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all ZNF536 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ZNF536 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ZNF536.The immunogen is located within amino acids 30 - 80 of ZNF536.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger protein 536

Cellular Localization

Nucleus

Background

ZNF536 is a recently identified zinc-finger protein that is expressed primarily in the developing nervous system and the cerebral cortex, hippocampus, and hypothalamus. ZNF536 possess ten zinc fingers and interacts with CtBP1, a corepressor for gene transcription. It is most closely related to transcriptional repressor ZNF219. Overexpression of ZNF536 in embryonic stem cells dramatically reduced the mRNA levels of neuronal marker genes such as Pax6, MAP2, and b-tubulin III following retinoic acid (RA)-induced differentiation, while depletion of ZNF536 via RNAi resulted in elevated mRNA levels of these genes, indicating its role in inhibiting neuronal cell differentiation. Overexpression of RA receptor a rescues the inhibitory role of ZNF536, suggesting that ZNF536 might inhibit RA response element-mediated transcriptional activity.

Database

Research Area

DATA IMAGES

Anti-ZNF536 antibody used in IHC (Paraffin sections) (IHC-P). GTX85225

GTX85225 IHC-P Image

IHC-P analysis of human brain tissue using GTX85225 ZNF536 antibody.
Working concentration : 20 μg/ml

Anti-ZNF536 antibody used in IHC (Paraffin sections) (IHC-P). GTX85225

GTX85225 IHC-P Image

IHC-P analysis of human brain tissue using GTX85225 ZNF536 antibody.
Working concentration : 5 μg/ml

Anti-ZNF536 antibody used in Western Blot (WB). GTX85225

GTX85225 WB Image

WB analysis of human brain tissue lysate using GTX85225 ZNF536 antibody.
Working concentration : 1 μg/ml

REFERENCE

Application Reference

Hu SH et al. Cell Discov 2021; 7 (1) : 103 Discovery of new genetic loci for male sexual orientation in Han population.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399