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ZNF550 antibody

Anti-ZNF550 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117305
Anti-ZNF550 antibody used in Western Blot (WB). GTX117305

Cat No. GTX117305

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

K562

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZNF550. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162864

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zinc finger protein 550

Cellular Localization

Nucleus

Background

May be involved in transcriptional regulation.

Database

DATA IMAGES

Anti-ZNF550 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117305

GTX117305 ICC/IF Image

ZNF550 antibody detects ZNF550 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF550 protein stained by ZNF550 antibody (GTX117305) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ZNF550 antibody used in Western Blot (WB). GTX117305

GTX117305 WB Image

Sample (30 ug of whole cell lysate)
A: K562
10% SDS PAGE
GTX117305 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169