Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ZNF571(GTX119554) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ZNF571 antibody [N1N2], N-term detects ZNF571 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF571 protein stained by ZNF571 antibody [N1N2], N-term (GTX119554) diluted at 1:500.
Blue: Hoechst 33342 staining.

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX119554 diluted at 1:3000