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ZNF598 antibody [N1N3]

Anti-ZNF598 antibody [N1N3] used in Western Blot (WB). GTX119245
Anti-ZNF598 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX119245
Anti-ZNF598 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119245
Anti-ZNF598 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX119245
Anti-ZNF598 antibody [N1N3] used in Western Blot (WB). GTX119245
Anti-ZNF598 antibody [N1N3] used in Western Blot (WB). GTX119245

Cat. No. GTX119245

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

Raji

Product Note

Knockdown/Knockout validation was supported by references (PMID:28065601)

Predict Reactivity

Zebrafish, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZNF598. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619017

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger protein 598 , HEL2

Database

Research Area

DATA IMAGES

Anti-ZNF598 antibody [N1N3] used in Western Blot (WB). GTX119245

GTX119245 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX119245 diluted at 1:1000

Anti-ZNF598 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX119245

GTX119245 IHC-P Image

ZNF598 antibody [N1N3] detects ZNF598 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
ZNF598 stained by ZNF598 antibody [N1N3] (GTX119245) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZNF598 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119245

GTX119245 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using ZNF598(GTX119245) antibody at 1:500 dilution.

Anti-ZNF598 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX119245

GTX119245 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ZNF598(GTX119245) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ZNF598 antibody [N1N3] used in Western Blot (WB). GTX119245

GTX119245 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZNF598 antibody [N1N3] (GTX119245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZNF598 antibody [N1N3] used in Western Blot (WB). GTX119245

GTX119245 WB Image

The data was published in the journal Nat Commun in 2017.PMID: 28685749

REFERENCE

REVIEW

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Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169