GeneTex
United States (US)

ZNF703 antibody

GTX107721_41115_20180720_WB_19011507_698.jpg
GTX107721_39757_WB_B_19011507_204.jpg
GTX107721_41115_20180706_WB_B_19011507_745.jpg
GTX107721_41115_IFA_19011507_626.jpg
GTX107721_39757_IHC_19011507_753.jpg

Cat No. GTX107721

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , ZNF703-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ZNF703. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zinc Finger Protein 703,Nlz1,Zeppo1,Znf503L,Zpo1,Znf703

Cellular Localization

Nucleus

Background

May function as a transcriptional repressor.

Database

DATA IMAGES
GTX107721_41115_20180720_WB_19011507_698.jpg

GTX107721 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ZNF703 antibody (GTX107721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107721_39757_WB_B_19011507_204.jpg

GTX107721 WB Image

Sample (30 ug of whole cell lysate)
A:  Non-transfected HeLa lysates 
B: ZNF703 transfected HeLa lysates
7.5% SDS PAGE
GTX107721 diluted at 1:5000

GTX107721_41115_20180706_WB_B_19011507_745.jpg

GTX107721 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ZNF703 antibody (GTX107721) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107721_41115_IFA_19011507_626.jpg

GTX107721 ICC/IF Image

ZNF703 antibody detects ZNF703 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF703 protein stained by ZNF703 antibody (GTX107721) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX107721_39757_IHC_19011507_753.jpg

GTX107721 IHC-P Image

ZNF703 antibody detects ZNF703 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ZNF703 antibody (GTX107721) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169