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ZNF703 antibody

Overexpression
Anti-ZNF703 antibody used in Western Blot (WB). GTX107721
Anti-ZNF703 antibody used in Western Blot (WB). GTX107721
Anti-ZNF703 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107721
Anti-ZNF703 antibody used in IHC (Paraffin sections) (IHC-P). GTX107721
Anti-ZNF703 antibody used in IHC (Paraffin sections) (IHC-P). GTX107721

Cat. No. GTX107721

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZNF703 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , ZNF703-transfected 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ZNF703. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952690

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger protein 703 , NLZ1 , ZEPPO1 , ZNF503L , ZPO1

Cellular Localization

Nucleus

Background

May function as a transcriptional repressor.

Database

Research Area

DATA IMAGES

Anti-ZNF703 antibody used in Western Blot (WB). GTX107721

GTX107721 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ZNF703 antibody (GTX107721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZNF703 antibody used in Western Blot (WB). GTX107721

GTX107721 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ZNF703 antibody (GTX107721) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZNF703 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107721

GTX107721 ICC/IF Image

ZNF703 antibody detects ZNF703 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF703 protein stained by ZNF703 antibody (GTX107721) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ZNF703 antibody used in IHC (Paraffin sections) (IHC-P). GTX107721

GTX107721 IHC-P Image

ZNF703 antibody detects ZNF703 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ZNF703 antibody (GTX107721) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ZNF703 antibody used in IHC (Paraffin sections) (IHC-P). GTX107721

GTX107721 IHC-P Image

The data was published in the journal Oncotarget in 2016. PMID: 27764785

REFERENCE

Application Reference

Udu-Ituma S et al. Pharmaceutics 2023; 15 (7) ZNF703 mRNA-Targeting Antisense Oligonucleotide Blocks Cell Proliferation and Induces Apoptosis in Breast Cancer Cell Lines.
Sandra Udu-Ituma et al. Pharmaceutics 2023; 15 (7) : 1930 ZNF703 mRNA-Targeting Antisense Oligonucleotide Blocks Cell Proliferation and Induces Apoptosis in Breast Cancer Cell Lines
Application : WB
Wang H et al. Cell Physiol Biochem 2017; 44 (3) : 920-934 Elevated Expression of Zinc Finger Protein 703 Promotes Cell Proliferation and Metastasis through PI3K/AKT/GSK-3β Signalling in Oral Squamous Cell Carcinoma.
Application : WB, IHC-P
Reactivity : Human
Li K et al. Oncotarget 2016; 7 (46) : 76108-76117 Overexpression of ZNF703 facilitates tumorigenesis and predicts unfavorable prognosis in patients with cholangiocarcinoma.
Application : IHC-P
Reactivity : Human
Springer S et al. Breast Cancer Res Treat 2015; 152 (2) : 313-21 Engineering targeted chromosomal amplifications in human breast epithelial cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169