GeneTex
United States (US)

ZNF74 antibody [C1C3]

GTX114892_40240_IFA_18121410_166.jpg
GTX114892_WB_18121410_690.jpg

Cat No. GTX114892

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZNF74. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zinc Finger Protein 74,Cos52,Zfp520,Znf520,Hznf7,Znf74

Cellular Localization

Nucleus

Background

May play a role in RNA metabolism.

Database

DATA IMAGES
GTX114892_40240_IFA_18121410_166.jpg

GTX114892 ICC/IF Image

ZNF74 antibody [C1C3] detects ZNF74 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF74 protein stained by ZNF74 antibody [C1C3] (GTX114892) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX114892_WB_18121410_690.jpg

GTX114892 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX114892 diluted at 1:5000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169