GeneTex
United States (US)

ZO-1 antibody [C3], C-term

GTX108587_40618_20160804_WB_shRNA_watermark_19011507_418.jpg
GTX108587_40618_WB_19011507_663.jpg
GTX108587_40618_WB_2_19011507_468.jpg

Cat No. GTX108587

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

195 kDa. ( Note )

Positive Control

HCT116 , 293T , A431 , HeLa

Predict Reactivity

Mouse, Rat, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ZO-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tight Junction Protein 1,Zo-1,Tjp1

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction

Background

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108587_40618_20160804_WB_shRNA_watermark_19011507_418.jpg

GTX108587 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [C3], C-term (GTX108587) diluted at 1:500.

GTX108587_40618_WB_19011507_663.jpg

GTX108587 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
5% SDS PAGE
GTX108587 diluted at 1:1000

GTX108587_40618_WB_2_19011507_468.jpg

GTX108587 WB Image

ZO-1 antibody detects ZO-1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
5 % SDS-PAGE
ZO-1 antibody (GTX108587) dilution: 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169