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ZO-1 antibody [HL1133]

Anti-ZO-1 antibody [HL1133] used in Western Blot (WB). GTX636399
Anti-ZO-1 antibody [HL1133] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636399
Anti-ZO-1 antibody [HL1133] used in Western Blot (WB). GTX636399

Cat No. GTX636399

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1133

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

195 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909975

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tight junction protein 1 , ZO-1

Cellular Localization

Cell membrane,Cell junction

Background

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-ZO-1 antibody [HL1133] used in Western Blot (WB). GTX636399

GTX636399 WB Image

Wild-type (WT) and TJP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1133] (GTX636399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [HL1133] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636399

GTX636399 ICC/IF Image

ZO-1 antibody [HL1133] detects ZO-1 protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody [HL1133] (GTX636399) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ZO-1 antibody [HL1133] used in Western Blot (WB). GTX636399

GTX636399 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1133] (GTX636399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for ZO-1 antibody [HL1133] (GTX636399). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ZO-1 antibody [HL1133] (GTX636399). Be the first to share your experience with this product.
Package List Price ($)
$ 379
$ 169
Custom Conjugations