APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909994
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tight junction protein 1 , ZO-1
Cellular Localization
Cell membrane,Cell junction
Background
This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491](/templates/default/js/lightbox/images/loading.gif "Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491")
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GTX636491 WB Image
Wild-type (WT) and TJP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (GTX636491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636491 ICC/IF Image
ZO-1 antibody [HL1185] detects ZO-1 protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ZO-1 stained by ZO-1 antibody [HL1185] (GTX636491) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636491 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (GTX636491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX636491 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (GTX636491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX636491 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (GTX636491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for ZO-1 antibody [HL1185] (GTX636491). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ZO-1 antibody [HL1185] (GTX636491). Be the first to share your experience with this product.
![Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491](/upload/website/prouct_img/small/GTX636491/GTX636491_44550_20220211_WB_KO_watermark_22021523_438.jpg "Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491")
![Anti-ZO-1 antibody [HL1185] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636491](/upload/website/prouct_img/small/GTX636491/GTX636491_T-44445_20211126_ICC_IF_21121501_431.jpg "Anti-ZO-1 antibody [HL1185] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636491")
![Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491](/upload/website/prouct_img/small/GTX636491/GTX636491_T-44445_20220107_WB_R_22011022_372.jpg "Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491")
![Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491](/upload/website/prouct_img/small/GTX636491/GTX636491_T-44445_20220107_WB_M_22011022_272.jpg "Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491")
![Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491](/upload/website/prouct_img/small/GTX636491/GTX636491_44550_20220107_WB_22011718_245.jpg "Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491")
![Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491](/upload/website/prouct_img/normal/GTX636491/GTX636491_44550_20220211_WB_KO_watermark_22021523_438.jpg "Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491")
![Anti-ZO-1 antibody [HL1185] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636491](/upload/website/prouct_img/normal/GTX636491/GTX636491_T-44445_20211126_ICC_IF_21121501_431.jpg "Anti-ZO-1 antibody [HL1185] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636491")
![Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491](/upload/website/prouct_img/normal/GTX636491/GTX636491_T-44445_20220107_WB_R_22011022_372.jpg "Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491")
![Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491](/upload/website/prouct_img/normal/GTX636491/GTX636491_T-44445_20220107_WB_M_22011022_272.jpg "Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491")
![Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491](/upload/website/prouct_img/normal/GTX636491/GTX636491_44550_20220107_WB_22011718_245.jpg "Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491")