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ZO-1 antibody [N1N2], N-term

KO/KD
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

Cat. No. GTX108613

Reference 23
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ZO-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

195 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952257

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tight junction protein 1 , ZO-1

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction

Background

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613

GTX108613 ICC/IF Image

ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: ZO-1 stained by ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613

GTX108613 ICC/IF Image

ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ZO-1 stained by ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500.

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wan-Ping Su et al. Biomed Pharmacother 2023; 163 Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition
Application : WB
Reactivity : Human
Russell DF et al. PLoS One 2022; 17 (11) : e0276854 Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon.
M?ckel M et al. Cells 2022; 11 (22) Human 3D Airway Tissue Models for Real-Time Microscopy: Visualizing Respiratory Virus Spreading.
Xun Liu et al. Cancer Sci 2022; 113 (5) : 1868-1879 Deubiquitinase ubiquitin-specific peptidase 10 maintains cysteine rich angiogenic inducer 61 expression via Yes1 associated transcriptional regulator to augment immune escape and metastasis of pancreatic adenocarcinoma.
Application : WB, ICC/IF
Reactivity : Human
Meng-Hui Zhang et al. Bioengineered 2022; 13 (4) : 10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.
Application : WB
Show More +
Submit a Reference

REVIEW

ZO-1 antibody [N1N2], N-term Cat. No. GTX108613

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Gastroenterol Res Pract in 2016. PMID: 27525003
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Biosci in 2019. PMID: 31114672
Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613
Date : Anonymous submitted on 31-Aug-2018
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Brain microvascular endothelial cell
Blocking : PBS with 0.1% Triton X-100, 5% goat serum
Primary Antibody : 1:50, 4°C, 16Hr
Submit a Review
Application Reference
Wan-Ping Su et al. Biomed Pharmacother 2023; 163 Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition
Application : WB
Reactivity : Human
Russell DF et al. PLoS One 2022; 17 (11):e0276854 Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon.
M?ckel M et al. Cells 2022; 11 (22) Human 3D Airway Tissue Models for Real-Time Microscopy: Visualizing Respiratory Virus Spreading.
Xun Liu et al. Cancer Sci 2022; 113 (5):1868-1879 Deubiquitinase ubiquitin-specific peptidase 10 maintains cysteine rich angiogenic inducer 61 expression via Yes1 associated transcriptional regulator to augment immune escape and metastasis of pancreatic adenocarcinoma.
Application : WB, ICC/IF
Reactivity : Human
Meng-Hui Zhang et al. Bioengineered 2022; 13 (4):10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.
Application : WB
Yu-Ting Yen et al. Int J Mol Sci 2021; 23 (1) Down-Regulation of miR-194-5p for Predicting Metastasis in Breast Cancer Cells.
Application : WB
Reactivity : Human
Russell DF et al. Front Neuroanat 2020; 14:50 Large-Scale Convergence of Receptor Cell Arrays Onto Afferent Terminal Arbors in the Lorenzinian Electroreceptors of Polyodon.
Voirin AC et al. Sci Rep 2020; 10 (1):11309 Sera of elderly obstructive sleep apnea patients alter blood-brain barrier integrity in vitro: a pilot study.
Reactivity : Human
Qu L et al. Front Pharmacol 2020; 11:918 Combination Compositions Composed of l-Glutamine and Si-Jun-Zi-Tang Might Be a Preferable Choice for 5-Fluorouracil-Induced Intestinal Mucositis: An Exploration in a Mouse Model.
Reactivity : Mouse
Tajik N et al. Nat Commun 2020; 11 (1):1995 Targeting zonulin and intestinal epithelial barrier function to prevent onset of arthritis.
Application : WB
Reactivity : Human
Yang P et al. Exp Biol Med (Maywood) 2019; 244 (18):1608-1618 ResolvinD1 attenuates high-mobility group box 1-induced epithelial-to-mesenchymal transition in nasopharyngeal carcinoma cells.
Yang P et al. Int J Med Sci 2019; 16 (12):1631-1641 Agonist of PPAR-γ Reduced Epithelial-Mesenchymal Transition in Eosinophilic Chronic Rhinosinusitis with Nasal Polyps via Inhibition of High Mobility Group Box1.
Boschetti E et al. Neurogastroenterol Motil 2019; 31 (8):e13652 Gut epithelial and vascular barrier abnormalities in patients with chronic intestinal pseudo-obstruction.
Xu G et al. Front Mol Neurosci 2019; 12:32 Restraint Stress Induced Hyperpermeability and Damage of the Blood-Brain Barrier in the Amygdala of Adult Rats.
Horioka K et al. Dig Dis Sci 2019; Acute Colchicine Poisoning Causes Endotoxemia via the Destruction of Intestinal Barrier Function: The Curative Effect of Endotoxin Prevention in a Murine Model.
Reactivity : Mouse
Xiao Ke et al. Int J Clin Exp Med 2019; 12 (6):6864-6873 The effects of Qing Hua Chang Yin on the epithelial tight junctions of mice with inflammatory bowel disease.
Application : WB
Reactivity : Mouse
Zhang N et al. Cell Biosci 2019; 9:40 Functional characterization of SLC26A3 c.392C>G (p.P131R) mutation in intestinal barrier function using CRISPR/CAS9-created cell models.
Application : WB
Reactivity : Human
Ding X et al. Lab Invest 2018; (Epub) SLC26A3 (DRA) prevents TNF-alpha-induced barrier dysfunction and dextran sulfate sodium-induced acute colitis.
Application : ICC/IF, IP
Reactivity : Human
Li H et al. Toxicol Appl Pharmacol 2017; 340:58-66 Astragaloside IV protects blood-brain barrier integrity from LPS-induced disruption via activating Nrf2 antioxidant signaling pathway in mice.
Application : WB
Reactivity : Mouse
Dominguez C et al. JCI Insight 2017; 2 (21) Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer.
Reactivity : Mouse
Chou HC et al. Toxicological Sciences 2017; (Epub) L-carnitine via PPARγ- and Sirt1-dependent mechanisms attenuates epithelial–mesenchymal transition and renal fibrosis caused by perfluorooctanesulfonate.
Application : WB
Arevalo-Romero H et al. Gastroenterol Res Pract 2016; 2016:4969163 Helicobacter pylori CagA and IL-1β Promote the Epithelial-to-Mesenchymal Transition in a Nontransformed Epithelial Cell Model.
Application : ICC/IF
Reactivity : Human
Chiu WC et al. PLoS One 2016; 11 (11):e0166453 The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169