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ZO-1 antibody [N1N2], N-term

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

Cat. No. GTX108613

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, IP

Reactivity

Polyodon spathula, Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

195 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952257

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tight junction protein 1 , ZO-1

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction

Background

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613

GTX108613 ICC/IF Image

ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ZO-1 stained by ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500.

Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613

GTX108613 ICC/IF Image

ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: ZO-1 stained by ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613

GTX108613 ICC/IF Image

The data was published in the journal Gastroenterol Res Pract in 2016. PMID: 27525003

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

The data was published in the journal Cell Biosci in 2019. PMID: 31114672

REFERENCE

REVIEW

ZO-1 antibody [N1N2], N-term Cat. No. GTX108613

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 31-Aug-2018
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Brain microvascular endothelial cell
Blocking : PBS with 0.1% Triton X-100, 5% goat serum
Primary Antibody : 1:50, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169