GeneTex

  • Country / Location Selection

United States (US)

ZO-1 antibody [N1N2], N-term

KO/KD
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

Cat. No. GTX108613

Reference 24
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IP

Reactivity

 Polyodon spathula, Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ZO-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

195 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952257

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tight junction protein 1 , ZO-1

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction

Background

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613

GTX108613 ICC/IF Image

ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ZO-1 stained by ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500.

Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613

GTX108613 ICC/IF Image

ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: ZO-1 stained by ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613

GTX108613 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Hsia Y et al. Int J Nanomedicine 2023; 18 : 4253-4274 A Dual Concentration-Tailored Cytokine-Chemo Nanosystem to Alleviate Multidrug Resistance and Redirect Balance of Cancer Proliferation and Apoptosis.
Wan-Ping Su et al. Biomed Pharmacother 2023; 163 Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition
Application : WB
Reactivity : Human
Russell DF et al. PLoS One 2022; 17 (11) : e0276854 Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon.
Application : IHC-Fr
Reactivity : Polyodon spathula
M?ckel M et al. Cells 2022; 11 (22) Human 3D Airway Tissue Models for Real-Time Microscopy: Visualizing Respiratory Virus Spreading.
Meng-Hui Zhang et al. Bioengineered 2022; 13 (4) : 10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.
Application : WB
Show More +
Submit a Reference

REVIEW

ZO-1 antibody [N1N2], N-term Cat. No. GTX108613

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Gastroenterol Res Pract in 2016. PMID: 27525003
Anti-ZO-1 antibody [N1N2], N-term used in Western Blot (WB). GTX108613
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Biosci in 2019. PMID: 31114672
Anti-ZO-1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108613
Date : Anonymous submitted on 31-Aug-2018
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Brain microvascular endothelial cell
Blocking : PBS with 0.1% Triton X-100, 5% goat serum
Primary Antibody : 1:50, 4°C, 16Hr
Submit a Review
Application Reference
Hsia Y et al. Int J Nanomedicine 2023; 18:4253-4274 A Dual Concentration-Tailored Cytokine-Chemo Nanosystem to Alleviate Multidrug Resistance and Redirect Balance of Cancer Proliferation and Apoptosis.
Wan-Ping Su et al. Biomed Pharmacother 2023; 163 Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition
Application : WB
Reactivity : Human
Russell DF et al. PLoS One 2022; 17 (11):e0276854 Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon.
Application : IHC-Fr
Reactivity : Polyodon spathula
M?ckel M et al. Cells 2022; 11 (22) Human 3D Airway Tissue Models for Real-Time Microscopy: Visualizing Respiratory Virus Spreading.
Xun Liu et al. Cancer Sci 2022; 113 (5):1868-1879 Deubiquitinase ubiquitin-specific peptidase 10 maintains cysteine rich angiogenic inducer 61 expression via Yes1 associated transcriptional regulator to augment immune escape and metastasis of pancreatic adenocarcinoma.
Application : WB, ICC/IF
Reactivity : Human
Meng-Hui Zhang et al. Bioengineered 2022; 13 (4):10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.
Application : WB
Yu-Ting Yen et al. Int J Mol Sci 2021; 23 (1) Down-Regulation of miR-194-5p for Predicting Metastasis in Breast Cancer Cells.
Application : WB
Reactivity : Human
Russell DF et al. Front Neuroanat 2020; 14:50 Large-Scale Convergence of Receptor Cell Arrays Onto Afferent Terminal Arbors in the Lorenzinian Electroreceptors of Polyodon.
Voirin AC et al. Sci Rep 2020; 10 (1):11309 Sera of elderly obstructive sleep apnea patients alter blood-brain barrier integrity in vitro: a pilot study.
Reactivity : Human
Qu L et al. Front Pharmacol 2020; 11:918 Combination Compositions Composed of l-Glutamine and Si-Jun-Zi-Tang Might Be a Preferable Choice for 5-Fluorouracil-Induced Intestinal Mucositis: An Exploration in a Mouse Model.
Reactivity : Mouse
Tajik N et al. Nat Commun 2020; 11 (1):1995 Targeting zonulin and intestinal epithelial barrier function to prevent onset of arthritis.
Application : WB
Reactivity : Human
Yang P et al. Exp Biol Med (Maywood) 2019; 244 (18):1608-1618 ResolvinD1 attenuates high-mobility group box 1-induced epithelial-to-mesenchymal transition in nasopharyngeal carcinoma cells.
Yang P et al. Int J Med Sci 2019; 16 (12):1631-1641 Agonist of PPAR-γ Reduced Epithelial-Mesenchymal Transition in Eosinophilic Chronic Rhinosinusitis with Nasal Polyps via Inhibition of High Mobility Group Box1.
Boschetti E et al. Neurogastroenterol Motil 2019; 31 (8):e13652 Gut epithelial and vascular barrier abnormalities in patients with chronic intestinal pseudo-obstruction.
Xu G et al. Front Mol Neurosci 2019; 12:32 Restraint Stress Induced Hyperpermeability and Damage of the Blood-Brain Barrier in the Amygdala of Adult Rats.
Horioka K et al. Dig Dis Sci 2019; Acute Colchicine Poisoning Causes Endotoxemia via the Destruction of Intestinal Barrier Function: The Curative Effect of Endotoxin Prevention in a Murine Model.
Reactivity : Mouse
Xiao Ke et al. Int J Clin Exp Med 2019; 12 (6):6864-6873 The effects of Qing Hua Chang Yin on the epithelial tight junctions of mice with inflammatory bowel disease.
Application : WB
Reactivity : Mouse
Zhang N et al. Cell Biosci 2019; 9:40 Functional characterization of SLC26A3 c.392C>G (p.P131R) mutation in intestinal barrier function using CRISPR/CAS9-created cell models.
Application : WB
Reactivity : Human
Ding X et al. Lab Invest 2018; (Epub) SLC26A3 (DRA) prevents TNF-alpha-induced barrier dysfunction and dextran sulfate sodium-induced acute colitis.
Application : ICC/IF, IP
Reactivity : Human
Li H et al. Toxicol Appl Pharmacol 2017; 340:58-66 Astragaloside IV protects blood-brain barrier integrity from LPS-induced disruption via activating Nrf2 antioxidant signaling pathway in mice.
Application : WB
Reactivity : Mouse
Dominguez C et al. JCI Insight 2017; 2 (21) Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer.
Reactivity : Mouse
Chou HC et al. Toxicological Sciences 2017; (Epub) L-carnitine via PPARγ- and Sirt1-dependent mechanisms attenuates epithelial–mesenchymal transition and renal fibrosis caused by perfluorooctanesulfonate.
Application : WB
Arevalo-Romero H et al. Gastroenterol Res Pract 2016; 2016:4969163 Helicobacter pylori CagA and IL-1β Promote the Epithelial-to-Mesenchymal Transition in a Nontransformed Epithelial Cell Model.
Application : ICC/IF
Reactivity : Human
Chiu WC et al. PLoS One 2016; 11 (11):e0166453 The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169