ZO-1 antibody [N2C1], Internal

Name: ZO-1 antibody [N2C1], Internal
Brand: GeneTex
SKU: GTX108627
Price: 399.00 USD
Rating: 4.5 (2 reviews)

Anti-ZO-1 antibody [N2C1], Internal used in Western Blot (WB). GTX108627

Anti-ZO-1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108627

Cat. No. GTX108627

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr
Reactivity	Human, Rat
Package	100 μl ($399), 25 μl ($169)

See all ZO-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-Fr	Assay dependent

Not tested in other applications.

Calculated MW

195 kDa. ( Note )

Positive Control

HeLa , H1299 , HCT-116

Predict Reactivity

Mouse, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZO-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731582

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tight junction protein 1 , ZO-1

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction

Background

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Gene ID: 7082 TJP1
UniProt: Q07157 TJP1

Research Area

DATA IMAGES

GTX108627 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal (GTX108627) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108627 ICC/IF Image

ZO-1 antibody [N2C1], Internal detects TJP1 protein at junction by confocal immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: TJP1 protein stained by ZO-1 antibody [N2C1], Internal (GTX108627) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

GTX108627 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal (GTX108627) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108627 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal (GTX108627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang HK et al. J Inflamm Res 2023; 16 : 4103-4116 HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis.

Wang HK et al. J Inflamm Res 2023; 2023 (16) : 4103—4116 HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis

Application : IHC-Fr

Reactivity : Rat

Takino JI et al. Sci Rep 2021; 11 (1) : 2959 RasGRP2 inhibits glyceraldehyde-derived toxic advanced glycation end-products from inducing permeability in vascular endothelial cells.

Application : WB, ICC/IF

Reactivity : Human

Michielin F et al. Cell Rep 2020; 33 (9) : 108453 The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs.

Application : ICC/IF

Reactivity : Human

Hung WT et al. Eur J Neurosci 2020; : e14896 Leptin protects brain from ischemia/reperfusion-induced infarction by stabilizing the blood-brain barrier to block brain infiltration by the blood-borne neutrophils.

Application : WB

Reactivity : Rat

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