GeneTex
United States (US)

ZO-1 antibody

KO/KD
GTX108592_40317_20160804_WB_shRNA_watermark_19011507_966.jpg
GTX108592_42760_20170322_IHC-P_M_19011507_966.jpg
GTX108592_42760_20170209_WB_19011507_651.jpg
GTX108592_40317_20141205_WB_2_19011507_721.jpg

Cat No. GTX108592

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000

Note :

WB
The use of GTX108592 for mouse is based on IHC-P testing. For WB using mouse samples, we recommend GTX108613

Not tested in other applications.

Calculated MW

195 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116

Predict Reactivity

Rat, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZO-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686M05161 antibody, MGC133289 antibody, ZO-1 antibody, TJP1 antibody, zona occludens protein 1 antibody, zona occludens 1 antibody, tight junction protein ZO-1 antibody, zonula occludens 1 protein antibody, zonula occludens protein 1 antibody, tight junction protein 1 (zona occludens 1) antibody

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction

Background

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108592_40317_20160804_WB_shRNA_watermark_19011507_966.jpg

GTX108592 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108592_42760_20170322_IHC-P_M_19011507_966.jpg

GTX108592 IHC-P Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ZO-1 antibody (GTX108592) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108592_42760_20170209_WB_19011507_651.jpg

GTX108592 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000.

GTX108592_40317_20141205_WB_2_19011507_721.jpg

GTX108592 WB Image

ZO-1 antibody detects ZO-1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169