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ZO-1 antibody

KO/KD Orthogonal
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592
Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

Cat. No. GTX108592

Reference 15
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Bovine, Dog, Pig, Monkey
Package
100 μl ($399),
25 μl ($169)
See all ZO-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent

Note :

WB
The use of GTX108592 for mouse is based on IHC-P testing. For WB using mouse samples, we recommend GTX108613
Not tested in other applications.

Calculated MW

195 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZO-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952260

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tight junction protein 1 , ZO-1

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction

Background

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592

GTX108592 IHC-P Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ZO-1 antibody (GTX108592) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000.

Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592

GTX108592 IHC-P Image

ZO-1 antibody detects ZO-1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592

GTX108592 ICC/IF Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:1000.

Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592

GTX108592 ICC/IF Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:1000.

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Peng-Hsiang Fang et al. Mol Med 2022; 28 (1) : 71 Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions.
Application : WB
Reactivity : Human
Tomi E.D. et al. Int J Mol Sci. 2022; 23 (3) : 1647 Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Wenyi Fang et al. Mol Med Rep 2021; 23 (3) : 1 Effects of Qing Hua Chang Yin on lipopolysaccharide?induced intestinal epithelial tight junction injury in Caco?2 cells.
Application : WB
Reactivity : Human
Yoshinobu Kariya et al. Commun Biol 2021; 4 (1) : 490 αvβ3 Integrin induces partial EMT independent of TGF-β signaling.
Application : WB, ICC/IF
Reactivity : Human
Barbian ME et al. Pediatr Res 2021; (Epub) Butyrate supplementation to pregnant mice elicits cytoprotection against colonic injury in the offspring.
Application : IHC-P
Show More +
Submit a Reference

REVIEW

ZO-1 antibody Cat. No. GTX108592

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592
Date : Anonymous submitted on 25-Oct-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Caco-2 cells
Blocking : 0.5% skim milk in TBST, RT, 1Hr
Fixation : 4%PFA/PBS, 15 min
Permeabilization /Antigen retrieval : TBST
Primary Antibody : 1:2000, 25°C, 12Hr
Anti-ZO-1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX108592
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Bov
Figure Note : The data was peer reviewed and published in the journal Proc Natl Acad Sci U S A in 2019. PMID: 31064871
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 26517522
Submit a Review
Application Reference
Peng-Hsiang Fang et al. Mol Med 2022; 28 (1):71 Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions.
Application : WB
Reactivity : Human
Tomi E.D. et al. Int J Mol Sci. 2022; 23 (3):1647 Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Wenyi Fang et al. Mol Med Rep 2021; 23 (3):1 Effects of Qing Hua Chang Yin on lipopolysaccharide?induced intestinal epithelial tight junction injury in Caco?2 cells.
Application : WB
Reactivity : Human
Yoshinobu Kariya et al. Commun Biol 2021; 4 (1):490 αvβ3 Integrin induces partial EMT independent of TGF-β signaling.
Application : WB, ICC/IF
Reactivity : Human
Watanabe D et al. Pharmaceutics 2021; 13 (9) Characterization of a Primate Blood-Brain Barrier Co-Culture Model Prepared from Primary Brain Endothelial Cells, Pericytes and Astrocytes.
Application : WB
Reactivity : Monkey
Barbian ME et al. Pediatr Res 2021; (Epub) Butyrate supplementation to pregnant mice elicits cytoprotection against colonic injury in the offspring.
Application : IHC-P
Minamide K et al. Sci Rep 2020; 10 (1):14639 IRF2 maintains the stemness of colonic stem cells by limiting physiological stress from interferon.
Application : IHC-P
Reactivity : Mouse
Bisi S et al. Nat Commun 2020; 11 (1):3516 IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules.
Xie MY et al. Biochem Pharmacol 2020; 175:113898 Porcine milk exosome miRNAs protect intestinal epithelial cells against deoxynivalenol-induced damage.
Application : WB
Reactivity : Pig
Kaliannan K et al. Commun Biol 2019; 2:276 Multi-omic analysis in transgenic mice implicates omega-6/omega-3 fatty acid imbalance as a risk factor for chronic disease.
Application : IHC-P
Reactivity : Mouse
Koch F et al. Proc Natl Acad Sci U S A 2019; 116 (21):10333-10338 Heat stress directly impairs gut integrity and recruits distinct immune cell populations into the bovine intestine.
Application : IHC-Fr
Reactivity : Bovine
Li W et al. J Clin Invest 2017; (Epub) The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.
Application : WB
Reactivity : Human
Jo H et al. Food Chem Toxicol 2017; 108 (Pt A):203-213 Oxyresveratrol improves tight junction integrity through the PKC and MAPK signaling pathways in Caco-2 cells.
Application : WB
Reactivity : Human
Sheng-Huei Yang et al. Oncotarget. 2016; 7 (1) Integrin β3 and LKB1 are independently involved in the inhibition of proliferation by lovastatin in human intrahepatic cholangiocarcinoma
Application : WB
Reactivity : Human
Hwang D et al. Biomed Pharmacother 2016; Conditioned medium from LS 174T goblet cells treated with oxyresveratrol strengthens tight junctions in Caco-2 cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169