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ZO-1 antibody

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

Cat No. GTX108592

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Bovine, Pig
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent

Note :

WB
The use of GTX108592 for mouse is based on IHC-P testing. For WB using mouse samples, we recommend GTX108613

Not tested in other applications.

Calculated MW

195 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZO-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952260

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tight junction protein 1 , ZO-1

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction

Background

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592

GTX108592 IHC-P Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ZO-1 antibody (GTX108592) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000.

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

ZO-1 antibody detects ZO-1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592

GTX108592 IHC-P Image

ZO-1 antibody detects ZO-1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

ZO-1 antibody Cat No. GTX108592

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Bov
Figure Note : The data was peer reviewed and published in the journal Proc Natl Acad Sci U S A in 2019.PMID: 31064871
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 26517522
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169