ZO-2 antibody

IHC-P analysis of human ovarian cancer tissue using GTX03355 TJP2 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

ICC/IF analysis of A431 cells using GTX03355 TJP2 antibody. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins.
Green : Primary antibody
Blue : DAPI
Dilution : 5μg/mL

FACS analysis of CACO-2 cells using GTX03355 TJP2 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

IHC-P analysis of human ovarian serous adenocarcinoma tissue using GTX03355 TJP2 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

IHC-P analysis of human rectal cancer tissue using GTX03355 TJP2 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

WB analysis of various sample lysates using GTX03355 TJP2 antibody.
Lane 1 : HEK293 whole cell lysates
Lane 2 : COS-7 whole cell lysates
Lane 3 : HeLa whole cell lysates
Lane 4 : MCF-7 whole cell lysates
Lane 5 : HepG2 whole cell lysates
Lane 6 : rat brain tissue lysates
Lane 7 : mouse brain tissue lysates
Lane 8 : HEPA1-6 whole cell lysates
Dilution : 0.5 μg/mL
Loading : 30μg per lane