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ZP2 antibody

Anti-ZP2 antibody used in Western Blot (WB). GTX117141
Anti-ZP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117141

Cat. No. GTX117141

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

IMR32

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human ZP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163348

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zona pellucida glycoprotein 2 , ZPA , Zp-2

Cellular Localization

Processed zona pellucida sperm-binding protein 2: Secreted , extracellular space , extracellular matrix , Cell membrane; Single-pass type I membrane protein

Background

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZP2 antibody used in Western Blot (WB). GTX117141

GTX117141 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX117141 diluted at 1:1000

Anti-ZP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117141

GTX117141 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using ZP2(GTX117141) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations