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ZPBP antibody

Anti-ZPBP antibody used in Western Blot (WB). GTX105073
Anti-ZPBP antibody used in IHC (Paraffin sections) (IHC-P). GTX105073
Anti-ZPBP antibody used in IHC (Paraffin sections) (IHC-P). GTX105073
Anti-ZPBP antibody used in IHC (Paraffin sections) (IHC-P). GTX105073

Cat No. GTX105073

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZPBP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

zona pellucida binding protein , ZPBP1

Cellular Localization

Secreted

Background

ZPBP is one of several proteins that are thought to participate in secondary binding between acrosome-reacted sperm and the egg-specific extracellular matrix, the zona pellucida (McLeskey et al., 1998 [PubMed 9378618]).[supplied by OMIM]

Database

DATA IMAGES
Anti-ZPBP antibody used in Western Blot (WB). GTX105073

GTX105073 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
B:HeLa S3(GTX14654)
12% SDS PAGE
GTX105073 diluted at 1:500

Anti-ZPBP antibody used in IHC (Paraffin sections) (IHC-P). GTX105073

GTX105073 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC25 xenograft, using ZPBP(GTX105073) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ZPBP antibody used in IHC (Paraffin sections) (IHC-P). GTX105073

GTX105073 IHC-P Image

ZPBP antibody detects secreted ZPBP protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
ZPBP stained by ZPBP antibody (GTX105073) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZPBP antibody used in IHC (Paraffin sections) (IHC-P). GTX105073

GTX105073 IHC-P Image

ZPBP antibody detects secreted ZPBP protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
ZPBP stained by ZPBP antibody (GTX105073) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169