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ZSCAN4 antibody

Anti-ZSCAN4 antibody used in IHC (Paraffin sections) (IHC-P). GTX85205
Anti-ZSCAN4 antibody used in Western Blot (WB). GTX85205
Anti-ZSCAN4 antibody used in IHC (Paraffin sections) (IHC-P). GTX85205

Cat. No. GTX85205

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all ZSCAN4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Mouse Lung Tissue Lysate(GTX27933)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ZSCAN4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZSCAN4.The immunogen is located within amino acids 370 - 420 of ZSCAN4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger and SCAN domain containing 4 , ZNF494

Cellular Localization

Nucleus

Background

Zytotic genome activation (ZGA) is the first and most critical transition event from the maternal genetic to the embryonic genetic program and the major burst of ZGA occurs only in the late 2-cell stage. ZSCAN4 encodes a SCAN domain and four zinc finger domains and is expressed in late 2-cell embryos and embryonic stem cells. Loss-of-function studies using siRNA shows delays in the progression from the 2-cell to the 4-cell stage and subsequent embryo development. ZSCAN4 plays an important role in the preimplantation development. Also, ZSCAN4 is involved in telomere maintenance and long term genomic stability in ES cells, suggesting that ZSCAN4 may be of critical importance in maintaining pluripotency in replicating stem cells.

Database

Research Area

DATA IMAGES

Anti-ZSCAN4 antibody used in IHC (Paraffin sections) (IHC-P). GTX85205

GTX85205 IHC-P Image

IHC-P analysis of rat lung tissue using GTX85205 ZSCAN4 antibody.
Working concentration : 5 μg/ml

Anti-ZSCAN4 antibody used in Western Blot (WB). GTX85205

GTX85205 WB Image

WB analysis of mouse lung tissue lysate in (A) the absence and (B) presence of blocking peptide using GTX85205 ZSCAN4 antibody.

Anti-ZSCAN4 antibody used in IHC (Paraffin sections) (IHC-P). GTX85205

GTX85205 IHC-P Image

IHC-P analysis of rat lung tissue using GTX85205 ZSCAN4 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399