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ZWILCH antibody

Anti-ZWILCH antibody used in Western Blot (WB). GTX116281
Anti-ZWILCH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116281
Anti-ZWILCH antibody used in IHC (Paraffin sections) (IHC-P). GTX116281

Cat. No. GTX116281

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZWILCH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZWILCH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619153

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zwilch kinetochore protein , KNTC1AP , hZwilch

Cellular Localization

Kinetochore

Background

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores.

Database

Research Area

DATA IMAGES

Anti-ZWILCH antibody used in Western Blot (WB). GTX116281

GTX116281 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX116281 diluted at 1:1000

Anti-ZWILCH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116281

GTX116281 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ZWILCH(GTX116281) antibody at 1:500 dilution.

Anti-ZWILCH antibody used in IHC (Paraffin sections) (IHC-P). GTX116281

GTX116281 IHC-P Image

ZWILCH antibody detects ZWILCH protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ZWILCH antibody (GTX116281) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169