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ZWINT antibody

Anti-ZWINT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107155
Anti-ZWINT antibody used in Western Blot (WB). GTX107155
Anti-ZWINT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107155

Cat. No. GTX107155

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZWINT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T , Molt-4 , Raji

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZWINT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952700

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ZW10 interacting kinetochore protein , HZwint-1 , KNTC2AP , SIP30 , ZWINT1

Cellular Localization

Nucleus , Kinetochore

Background

This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZWINT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107155

GTX107155 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed 293T, using ZWINT(GTX107155) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-ZWINT antibody used in Western Blot (WB). GTX107155

GTX107155 WB Image

Sample(30 ug whole cell lysate)
A:293T
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
12% SDS PAGE
GTX107155 diluted at 1:1000

Anti-ZWINT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107155

GTX107155 ICC/IF Image

ZWINT antibody detects ZWINT protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZWINT protein stained by ZWINT antibody (GTX107155) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Kim JH et al. J Cancer 2020; 11 (19) : 5601-5611 Involvement of the NF-κB signaling pathway in proliferation and invasion inhibited by Zwint-1 deficiency in Pancreatic Cancer Cells.
Application : WB
Reactivity : Human
Zhou H et al. Nat Commun 2016; 7 : 10151 Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.
Application : ICC/IF
Reactivity : Human
Wei R et al. Mol Biol Cell 2011; 22 (19) : 3584-94 Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169