GeneTex
United States (US)

Zika virus Capsid protein antibody

GTX133317_42921_20170714_WB_Zika_18121410_325.jpg
GTX133317_42921_20170810_WB_B_18121410_710.jpg
GTX133317_42921_20170908_WB_B_Zika_18121410_757.jpg
GTX133317_42921_20170911_IFA_18121410_895.jpg
GTX133317_42921_20170629_WB_Zika_18121410_933.jpg

Cat No. GTX133317

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Zika virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Zika virus Capsid-transfected 293T , Zika-infected Vero whole cell extracts , Zika Virus-PRVABC59 infected C6/36 cells , Zika virus Capsid-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Full length Zika virus Capsid recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zika virus Capsid antibody

Research Area

DATA IMAGES
GTX133317_42921_20170714_WB_Zika_18121410_325.jpg

GTX133317 WB Image

Mock and infected Vero whole cell extracts (20 µg) were separated by 4-20% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein (GTX133317) diluted at 1:2500.

GTX133317_42921_20170810_WB_B_18121410_710.jpg

GTX133317 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 µg) were separated by 15% SDS-PAGE, and the membranes were blotted with Zika virus Capsid protein antibody (GTX133317) diluted at 1:5000 and DDDDK tag antibody (GTX115043) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133317_42921_20170908_WB_B_Zika_18121410_757.jpg

GTX133317 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein (GTX133317) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133317_42921_20170911_IFA_18121410_895.jpg

GTX133317 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus Capsid protein antibody (GTX133317).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus Capsid protein antibody (GTX133317) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX133317_42921_20170629_WB_Zika_18121410_933.jpg

GTX133317 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extract (20 µg) were separated by 4-20% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein (GTX133317) diluted at 1:10000.

REFERENCE
REVIEW

Zika virus Capsid protein antibody Cat No. GTX133317

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected vero cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 359
$ 169