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Zika virus Capsid protein antibody

Orthogonal
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317
Anti-Zika virus Capsid protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133317
Anti-Zika virus Capsid protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133317
Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317

Cat. No. GTX133317

Reference 31
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus Capsid protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Zika Virus-PRVABC59 infected C6/36 cells , Zika-infected Vero extracts , Zika virus Capsid-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Full length Zika virus Capsid recombinant protein. (Zika virus (strain H/PF/2013))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2756861

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Capsid , ZIKV Capsid

Research Area

DATA IMAGES

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Mock and infected Vero whole cell extracts (20 μg) were separated by 4-20% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein (GTX133317) diluted at 1:2500.

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extract (20 μg) were separated by 4-20% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein (GTX133317) diluted at 1:10000.

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein antibody (GTX133317) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Zika virus Capsid protein antibody (GTX133317) diluted at 1:5000 and DDDDK tag antibody (GTX115043) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317

GTX133317 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus Capsid protein antibody (GTX133317).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus Capsid protein antibody (GTX133317) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Zika virus Capsid protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133317

GTX133317 IHC-P (cell pellet) Image

Zika virus Capsid antibody detects Zika virus Capsid protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Zika virus Capsid transfected 293T cell pellet.
Green: Zika virus Capsid stained by Zika virus Capsid antibody (GTX133317) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Zika virus Capsid protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133317

GTX133317 IHC-P (cell pellet) Image

Zika virus Capsid antibody detects Zika virus Capsid protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Zika virus Capsid transfected 293T cell pellet.
Green: Zika virus Capsid stained by Zika virus Capsid antibody (GTX133317) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317

GTX133317 ICC/IF Image

Immunofluorescent analysis of mock and Zika virus-infected cells using Zika virus Capsid protein antibodyantibody (GTX133317).
Sample: Zika virus non-infected and infected cells slide.
Green: Zika virus Capsid protein antibodyantibody (GTX133317) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Bollman B et al. NPJ Vaccines 2023; 8 (1) : 58 An optimized messenger RNA vaccine candidate protects non-human primates from Zika virus infection.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Fu Y et al. PLoS Pathog 2023; 19 (1) : e1011090 Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropism.
Application : WB
Rangel MV et al. J Virol 2022; 96 (2) : e0177421 Structurally Conserved Domains between Flavivirus and Alphavirus Fusion Glycoproteins Contribute to Replication and Infectious-Virion Production.
Trinh QD et al. Cells 2022; 11 (19) TGF-β1 Promotes Zika Virus Infection in Immortalized Human First-Trimester Trophoblasts via the Smad Pathway.
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Submit a Reference

REVIEW

Zika virus Capsid protein antibody Cat. No. GTX133317

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 29895962
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected vero cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, 16Hr
Submit a Review
Application Reference
Bollman B et al. NPJ Vaccines 2023; 8 (1):58 An optimized messenger RNA vaccine candidate protects non-human primates from Zika virus infection.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Fu Y et al. PLoS Pathog 2023; 19 (1):e1011090 Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropism.
Application : WB
Jung HG et al. Emerg Microbes Infect 2022; 11 (1):2447-2465 Influence of Zika virus 3'-end sequence and nonstructural protein evolution on the viral replication competence and virulence.
Rangel MV et al. J Virol 2022; 96 (2):e0177421 Structurally Conserved Domains between Flavivirus and Alphavirus Fusion Glycoproteins Contribute to Replication and Infectious-Virion Production.
Trinh QD et al. Cells 2022; 11 (19) TGF-β1 Promotes Zika Virus Infection in Immortalized Human First-Trimester Trophoblasts via the Smad Pathway.
Li S et al. J Virol 2022;:e0184621 Tracking the Replication-Competent Zika Virus with Tetracysteine-Tagged Capsid Protein in Living Cells.
Xi Yu et al. Proc Natl Acad Sci U S A 2021; 118 (42) A mutation-mediated evolutionary adaptation of Zika virus in mosquito and mammalian host.
Application : WB
Sara B York et al. mSphere 2021;:e0019221 Zika Virus Hijacks Extracellular Vesicle Tetraspanin Pathways for Cell-to-Cell Transmission.
Application : WB, ICC/IF
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12):3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Zhang Y et al. Emerg Microbes Infect 2020; 9 (1):1580-1589 The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals.
Clark AE et al. J Virol 2020; (Epub) Zika virus is transmitted in neural progenitor cells via cell-to-cell spread and infection is inhibited by the autophagy inducer trehalose.
Application : WB
Tan TY et al. Nat Commun 2020; 11 (1):895 Capsid protein structure in Zika virus reveals the flavivirus assembly process.
Kim M et al. Biomaterials 2020; 263:120391 Antiviral efficacy of orally delivered neoagarohexaose, a nonconventional TLR4 agonist, against norovirus infection in mice.
Zeng J et al. Cell Stem Cell 2020; 27 (4):618-632.e9 The Zika Virus Capsid Disrupts Corticogenesis by Suppressing Dicer Activity and miRNA Biogenesis.
Application : WB, ICC/IF
Chen J et al. mBio 2020; 11 (5) Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
Application : ICC/IF
Wang Y et al. Viruses 2020; 12 (5) TRiC/CCT Complex, a Binding Partner of NS1 Protein, Supports the Replication of Zika Virus in Both Mammalians and Mosquitoes.
Application : WB
Garg H et al. Sci Rep 2020; 10 (1):4017 Virus Like Particles (VLP) as multivalent vaccine candidate against Chikungunya, Japanese Encephalitis, Yellow Fever and Zika Virus.
Application : WB
Li M et al. Nat Microbiol 2019; 4 (6):985-995 Identification of antiviral roles for the exon-junction complex and nonsense-mediated decay in flaviviral infection.
Garg H et al. Vaccine 2019; Capsid containing virus like particle vaccine against Zika virus made from a stable cell line.
Application : WB
Wang Y et al. J Med Virol 2019; Inactivation of Zika Virus in plasma and derivatives by four different methods.
Application : ICC/IF
Li PC et al. ACS Infect Dis 2019; (Epub) Small molecules targeting the flavivirus E protein with broad-spectrum activity and antiviral efficacy in vivo.
Taguwa S et al. Cell Rep 2019; 26 (4):906-920.e3 Zika Virus Dependence on Host Hsp70 Provides a Protective Strategy against Infection and Disease.
Application : WB
Gaston Bonenfant et al. bioRxiv 2018; (Epub) Zika Virus Subverts Stress Granules to Promote and Restrict Viral Gene Expression.
Application : WB
Scaturro P et al. Nature 2018; 561 (7722):253-257 An orthogonal proteomic survey uncovers novel Zika virus host factors.
Application : WB
Fink SL et al. Sci Rep 2018; 8 (1):8989 The Antiviral Drug Arbidol Inhibits Zika Virus.
Application : WB
Kuo YP et al. PLoS Negl Trop Dis 2018; 12 (4):e0006417 Establishment of a mouse model for the complete mosquito-mediated transmission cycle of Zika virus.
Application : WB
McIntyre W et al. Nucleic Acids Res 2018; (Epub) Positive-sense RNA viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection.
Application : WB
Cheng F et al. J Virol 2017; (Epub) Suppression of Zika virus infection and replication in endothelial cells and astrocytes by PKA inhibitor PKI 14-22.
Application : WB
Khaiboullina SF et al. Sci Rep 2017; 7 (1):16050 ZIKV infection regulates inflammasomes pathway for replication in monocytes.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169