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Zika virus Capsid protein antibody

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

Cat No. GTX133317

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Zika virus
Package
100 μl ($359),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Zika virus Capsid-transfected 293T , Zika-infected Vero whole cell extracts , Zika Virus-PRVABC59 infected C6/36 cells , Zika virus Capsid-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Full length Zika virus Capsid recombinant protein. (Zika virus (strain H/PF/2013))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2756861

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Capsid , ZIKV Capsid

Research Area

DATA IMAGES

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Mock and infected Vero whole cell extracts (20 μg) were separated by 4-20% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein (GTX133317) diluted at 1:2500.

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Zika virus Capsid protein antibody (GTX133317) diluted at 1:5000 and DDDDK tag antibody (GTX115043) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein (GTX133317) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317

GTX133317 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus Capsid protein antibody (GTX133317).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus Capsid protein antibody (GTX133317) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extract (20 μg) were separated by 4-20% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein (GTX133317) diluted at 1:10000.

REFERENCE

REVIEW

Zika virus Capsid protein antibody Cat No. GTX133317

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29895962
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected vero cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf