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Zika virus Capsid protein antibody

Orthogonal
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317
Anti-Zika virus Capsid protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

Cat. No. GTX133317

Reference 35
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus Capsid protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Zika Virus-PRVABC59 infected C6/36 cells , Zika-infected Vero extracts , Zika virus Capsid-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Full length Zika virus Capsid recombinant protein. (Zika virus (strain H/PF/2013))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2756861

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Capsid , ZIKV Capsid

Research Area

DATA IMAGES

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Mock and infected Vero whole cell extracts (20 μg) were separated by 4-20% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein (GTX133317) diluted at 1:2500.

Anti-Zika virus Capsid protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133317

GTX133317 IHC-P (cell pellet) Image

Zika virus Capsid antibody detects Zika virus Capsid protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Zika virus Capsid transfected 293T cell pellet.
Green: Zika virus Capsid stained by Zika virus Capsid antibody (GTX133317) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Zika virus Capsid protein antibody (GTX133317) diluted at 1:5000 and DDDDK tag antibody (GTX115043) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317

GTX133317 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus Capsid protein antibody (GTX133317).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus Capsid protein antibody (GTX133317) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (5 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein antibody (GTX133317) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein antibody (GTX133317) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317

GTX133317 ICC/IF Image

Immunofluorescent analysis of mock and Zika virus-infected cells using Zika virus Capsid protein antibodyantibody (GTX133317).
Sample: Zika virus non-infected and infected cells slide.
Green: Zika virus Capsid protein antibodyantibody (GTX133317) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Zika virus Capsid protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133317

GTX133317 IHC-P (cell pellet) Image

Zika virus Capsid antibody detects Zika virus Capsid protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Zika virus Capsid transfected 293T cell pellet.
Green: Zika virus Capsid stained by Zika virus Capsid antibody (GTX133317) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2018. PMID: 29895962

REFERENCE

Application Reference

Lu AY et al. J Virol 2023; 97 (5) : e0198222 Viral Protein Accumulation of Zika Virus Variants Links with Regulation of Innate Immunity for Differential Control of Viral Replication, Spread, and Response to Interferon.
Song GY et al. Nat Commun 2023; 14 (1) : 6832 A single amino acid substitution in the capsid protein of Zika virus contributes to a neurovirulent phenotype.
He J et al. Viruses 2023; 15 (6) Zika Virus Induces Degradation of the Numb Protein Required through Embryonic Neurogenesis.
Bollman B et al. NPJ Vaccines 2023; 8 (1) : 58 An optimized messenger RNA vaccine candidate protects non-human primates from Zika virus infection.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Show More +
Submit a Reference

REVIEW

Zika virus Capsid protein antibody Cat. No. GTX133317

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected vero cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, 16Hr
Submit a Review
Application Reference
Lu AY et al. J Virol 2023; 97 (5):e0198222 Viral Protein Accumulation of Zika Virus Variants Links with Regulation of Innate Immunity for Differential Control of Viral Replication, Spread, and Response to Interferon.
Song GY et al. Nat Commun 2023; 14 (1):6832 A single amino acid substitution in the capsid protein of Zika virus contributes to a neurovirulent phenotype.
He J et al. Viruses 2023; 15 (6) Zika Virus Induces Degradation of the Numb Protein Required through Embryonic Neurogenesis.
Bollman B et al. NPJ Vaccines 2023; 8 (1):58 An optimized messenger RNA vaccine candidate protects non-human primates from Zika virus infection.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Fu Y et al. PLoS Pathog 2023; 19 (1):e1011090 Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropism.
Application : WB
Yu J et al. J Med Virol 2022; 94 (11):5392-5400 Development and characterization of an inducible assay system to measure Zika virus capsid interactions.
Jung HG et al. Emerg Microbes Infect 2022; 11 (1):2447-2465 Influence of Zika virus 3'-end sequence and nonstructural protein evolution on the viral replication competence and virulence.
Rangel MV et al. J Virol 2022; 96 (2):e0177421 Structurally Conserved Domains between Flavivirus and Alphavirus Fusion Glycoproteins Contribute to Replication and Infectious-Virion Production.
Trinh QD et al. Cells 2022; 11 (19) TGF-β1 Promotes Zika Virus Infection in Immortalized Human First-Trimester Trophoblasts via the Smad Pathway.
Li S et al. J Virol 2022;:e0184621 Tracking the Replication-Competent Zika Virus with Tetracysteine-Tagged Capsid Protein in Living Cells.
Xi Yu et al. Proc Natl Acad Sci U S A 2021; 118 (42) A mutation-mediated evolutionary adaptation of Zika virus in mosquito and mammalian host.
Application : WB
Sara B York et al. mSphere 2021;:e0019221 Zika Virus Hijacks Extracellular Vesicle Tetraspanin Pathways for Cell-to-Cell Transmission.
Application : WB, ICC/IF
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12):3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Zhang Y et al. Emerg Microbes Infect 2020; 9 (1):1580-1589 The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals.
Clark AE et al. J Virol 2020; (Epub) Zika virus is transmitted in neural progenitor cells via cell-to-cell spread and infection is inhibited by the autophagy inducer trehalose.
Application : WB
Tan TY et al. Nat Commun 2020; 11 (1):895 Capsid protein structure in Zika virus reveals the flavivirus assembly process.
Kim M et al. Biomaterials 2020; 263:120391 Antiviral efficacy of orally delivered neoagarohexaose, a nonconventional TLR4 agonist, against norovirus infection in mice.
Zeng J et al. Cell Stem Cell 2020; 27 (4):618-632.e9 The Zika Virus Capsid Disrupts Corticogenesis by Suppressing Dicer Activity and miRNA Biogenesis.
Application : WB, ICC/IF
Chen J et al. mBio 2020; 11 (5) Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
Application : ICC/IF
Wang Y et al. Viruses 2020; 12 (5) TRiC/CCT Complex, a Binding Partner of NS1 Protein, Supports the Replication of Zika Virus in Both Mammalians and Mosquitoes.
Application : WB
Garg H et al. Sci Rep 2020; 10 (1):4017 Virus Like Particles (VLP) as multivalent vaccine candidate against Chikungunya, Japanese Encephalitis, Yellow Fever and Zika Virus.
Application : WB
Li M et al. Nat Microbiol 2019; 4 (6):985-995 Identification of antiviral roles for the exon-junction complex and nonsense-mediated decay in flaviviral infection.
Garg H et al. Vaccine 2019; Capsid containing virus like particle vaccine against Zika virus made from a stable cell line.
Application : WB
Wang Y et al. J Med Virol 2019; Inactivation of Zika Virus in plasma and derivatives by four different methods.
Application : ICC/IF
Li PC et al. ACS Infect Dis 2019; (Epub) Small molecules targeting the flavivirus E protein with broad-spectrum activity and antiviral efficacy in vivo.
Taguwa S et al. Cell Rep 2019; 26 (4):906-920.e3 Zika Virus Dependence on Host Hsp70 Provides a Protective Strategy against Infection and Disease.
Application : WB
Gaston Bonenfant et al. bioRxiv 2018; (Epub) Zika Virus Subverts Stress Granules to Promote and Restrict Viral Gene Expression.
Application : WB
Scaturro P et al. Nature 2018; 561 (7722):253-257 An orthogonal proteomic survey uncovers novel Zika virus host factors.
Application : WB
Fink SL et al. Sci Rep 2018; 8 (1):8989 The Antiviral Drug Arbidol Inhibits Zika Virus.
Application : WB
Kuo YP et al. PLoS Negl Trop Dis 2018; 12 (4):e0006417 Establishment of a mouse model for the complete mosquito-mediated transmission cycle of Zika virus.
Application : WB
McIntyre W et al. Nucleic Acids Res 2018; (Epub) Positive-sense RNA viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection.
Application : WB
Cheng F et al. J Virol 2017; (Epub) Suppression of Zika virus infection and replication in endothelial cells and astrocytes by PKA inhibitor PKI 14-22.
Application : WB
Khaiboullina SF et al. Sci Rep 2017; 7 (1):16050 ZIKV infection regulates inflammasomes pathway for replication in monocytes.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169