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Zika virus Capsid protein antibody

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317
Anti-Zika virus Capsid protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133317
Anti-Zika virus Capsid protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317
Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

Cat. No. GTX133317

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P (cell pellet)

Reactivity

Zika virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Zika Virus-PRVABC59 infected C6/36 cells , Zika-infected Vero extracts , Zika virus Capsid-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Full length Zika virus Capsid recombinant protein. (Zika virus (strain H/PF/2013))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2756861

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Capsid , ZIKV Capsid

Research Area

DATA IMAGES

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Mock and infected Vero whole cell extracts (20 μg) were separated by 4-20% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein (GTX133317) diluted at 1:2500.

Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317

GTX133317 ICC/IF Image

Immunofluorescent analysis of mock and Zika virus-infected cells using Zika virus Capsid protein antibodyantibody (GTX133317).
Sample: Zika virus non-infected and infected cells slide.
Green: Zika virus Capsid protein antibodyantibody (GTX133317) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Zika virus Capsid protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133317

GTX133317 IHC-P (cell pellet) Image

Zika virus Capsid antibody detects Zika virus Capsid protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Zika virus Capsid transfected 293T cell pellet.
Green: Zika virus Capsid stained by Zika virus Capsid antibody (GTX133317) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Zika virus Capsid protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133317

GTX133317 IHC-P (cell pellet) Image

Zika virus Capsid antibody detects Zika virus Capsid protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Zika virus Capsid transfected 293T cell pellet.
Green: Zika virus Capsid stained by Zika virus Capsid antibody (GTX133317) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (5 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein antibody (GTX133317) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Zika virus Capsid protein antibody (GTX133317) diluted at 1:5000 and DDDDK tag antibody (GTX115043) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133317

GTX133317 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus Capsid protein antibody (GTX133317).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus Capsid protein antibody (GTX133317) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus Capsid protein antibody (GTX133317) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Capsid protein antibody used in Western Blot (WB). GTX133317

GTX133317 WB Image

The data was published in the journal Sci Rep in 2018. PMID: 29895962

REFERENCE

REVIEW

Zika virus Capsid protein antibody Cat. No. GTX133317

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected vero cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169