Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133325 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus Envelope protein antibody (GTX133325).
Green: Zika virus Envelope protein antibody (GTX133325) diluted at 1:500.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634157 as capture antibody at concentration of 5 μg/mL and GTX133325 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133325) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mock and infected Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133325) diluted at 1:2500.

Immunofluorescent analysis of mock and Zika virus-infected cells using Zika virus Envelope protein antibodyantibody (GTX133325).
Sample: Zika virus non-infected and infected cells slide.
Green: Zika virus Envelope protein antibodyantibody (GTX133325) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

The data was published in the journal Sci Rep in 2018. PMID: 29895962