GeneTex

  • Country / Location Selection

United States (US)

Zika virus Envelope protein antibody

Orthogonal
Anti-Zika virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133325
Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325
Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325
Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133325
Anti-Zika virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133325
Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325
Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133325
Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325

Cat. No. GTX133325

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus Envelope protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634157 / GTX634155, Detection : GTX133325
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Zika-infected Vero , Zika virus Envelope-transfected 293T , Zika virus Envelope-infected C6/36

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Zika virus Envelope protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886930

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Envelope , ZIKV Envelope

Research Area

DATA IMAGES

Anti-Zika virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133325

GTX133325 ICC/IF Image

Immunofluorescent analysis of mock and Zika virus-infected cells using Zika virus Envelope protein antibodyantibody (GTX133325).
Sample: Zika virus non-infected and infected cells slide.
Green: Zika virus Envelope protein antibodyantibody (GTX133325) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325

GTX133325 WB Image

Mock and infected Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133325) diluted at 1:2500.

Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325

GTX133325 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133325) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133325

GTX133325 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634157 as capture antibody at concentration of 5 μg/mL and GTX133325 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133325

GTX133325 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus Envelope protein antibody (GTX133325).
Green: Zika virus Envelope protein antibody (GTX133325) diluted at 1:500.

Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325

GTX133325 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133325

GTX133325 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133325 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325

GTX133325 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2018. PMID: 29895962

REFERENCE

Application Reference

Wu H et al. Nat Commun 2023; 14 (1) : 5541 Zika virus targets human trophoblast stem cells and prevents syncytialization in placental trophoblast organoids.
BR Sahoo et al. J Virol 2020; 94 (22) : e01575-20 Mechanistic Target of Rapamycin Signaling Activation Antagonizes Autophagy To Facilitate Zika Virus Replication
Application : WB
Kabir MA et al. Sci Rep 2020; 10 (1) : 11906 An antibody panel for highly specific detection and differentiation of Zika virus.
Fink SL et al. Sci Rep 2018; 8 (1) : 8989 The Antiviral Drug Arbidol Inhibits Zika Virus.
Application : WB
Submit a Reference

REVIEW

There are currently no reviews for Zika virus Envelope protein antibody (GTX133325). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169