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Zika virus NS1 protein antibody [GT5212]

Anti-Zika virus NS1 protein antibody [GT5212] used in Western Blot (WB). GTX634158
Anti-Zika virus NS1 protein antibody [GT5212] used in Western Blot (WB). GTX634158
Anti-Zika virus NS1 protein antibody [GT5212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634158
Anti-Zika virus NS1 protein antibody [GT5212] used in Western Blot (WB). GTX634158
Anti-Zika virus NS1 protein antibody [GT5212] used in ELISA (ELISA). GTX634158
Anti-Zika virus NS1 protein antibody [GT5212] used in ELISA (ELISA). GTX634158
Anti-Zika virus NS1 protein antibody [GT5212] used in ELISA (ELISA). GTX634158
Anti-Zika virus NS1 protein antibody [GT5212] used in ELISA (ELISA). GTX634158
Anti-Zika virus NS1 protein antibody [GT5212] used in ELISA (ELISA). GTX634158

Cat No. GTX634158

Host

Mouse

Clonality

Monoclonal

Clone Name

GT5212

Isotype

IgG1

Application

WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

Zika virus
Package
100 μl ($359),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634158/GTX133324, Detection: GTX133323/GTX133307/GTX133324/GTX133306/GTX634158

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Zika-infected Vero whole cell extracts , Zika virus NS1-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Zika virus NS1 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888409

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Non-structural protein 1 , Zika virus NS1 , ZIKV Non-structural protein 1 , ZIKV NS1

Research Area

DATA IMAGES

Anti-Zika virus NS1 protein antibody [GT5212] used in Western Blot (WB). GTX634158

GTX634158 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody [GT5212] (GTX634158) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody [GT5212] used in Western Blot (WB). GTX634158

GTX634158 WB Image

Mock and infected Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody [GT5212] (GTX634158) diluted at 1:2500.

Anti-Zika virus NS1 protein antibody [GT5212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634158

GTX634158 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus NS1 protein antibody [GT5212] (GTX634158).
Green: Zika virus NS1 protein antibody [GT5212] (GTX634158) diluted at 1:500.

Anti-Zika virus NS1 protein antibody [GT5212] used in Western Blot (WB). GTX634158

GTX634158 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody [GT5212] (GTX634158) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody [GT5212] used in ELISA (ELISA). GTX634158

GTX634158 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133307 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody [GT5212] used in ELISA (ELISA). GTX634158

GTX634158 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133324 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody [GT5212] used in ELISA (ELISA). GTX634158

GTX634158 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX133324 as capture antibody at concentration of 5 μg/mL and GTX634158 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody [GT5212] used in ELISA (ELISA). GTX634158

GTX634158 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133323 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody [GT5212] used in ELISA (ELISA). GTX634158

GTX634158 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133306 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf