Zika virus NS1 protein antibody [GT5212]

Cat. No. GTX634158

Reference 4

Host	Mouse
Clonality	Monoclonal
Clone Name	GT5212
Isotype	IgG1
Application	WB, ICC/IF, ELISA, Sandwich ELISA
Reactivity	Zika virus
Package	100 μl ($399), 25 μl ($169)

See all Zika virus NS1 protein products

View product citations for antibody GTX634158 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
ELISA	1:1000-1:10000
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX634158 / GTX133324, Detection : GTX133323 / GTX133307 / GTX133324 / GTX133306 / GTX634158

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Zika-infected Vero extracts

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Zika virus NS1 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888409

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 1 , Zika virus NS1 , ZIKV Non-structural protein 1 , ZIKV NS1

Research Area

DATA IMAGES

GTX634158 ICC/IF Image

Immunofluorescent analysis of mock and Zika virus-infected cells using Zika virus NS1 protein antibody [GT5212] (GTX634158).
Sample: Zika virus non-infected and infected cells slide.
Green: Zika virus NS1 protein antibody [GT5212] (GTX634158) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

GTX634158 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX133324 as capture antibody at concentration of 5 μg/mL and GTX634158 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634158 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus NS1 protein antibody [GT5212] (GTX634158).
Green: Zika virus NS1 protein antibody [GT5212] (GTX634158) diluted at 1:500.

GTX634158 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133306 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634158 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133324 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634158 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody [GT5212] (GTX634158) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX634158 WB Image

Mock and infected Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody [GT5212] (GTX634158) diluted at 1:2500.

GTX634158 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133307 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634158 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133323 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634158 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody [GT5212] (GTX634158) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Laura Riva et al. J Virol 2021; 95 (22) : e0099621 The Compound SBI-0090799 Inhibits Zika Virus Infection by Blocking De Novo Formation of the Membranous Replication Compartment.

Application : WB

Sarah Goellner et al. STAR Protoc 2020; 1 (3) : 100173 Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.

Application : WB, ICC/IF

Cerikan B et al. Cell Rep 2020; 32 (1) : 107859 A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.

Application : ICC/IF

Wang Y et al. Viruses 2020; 12 (5) TRiC/CCT Complex, a Binding Partner of NS1 Protein, Supports the Replication of Zika Virus in Both Mammalians and Mosquitoes.

Application : ICC/IF

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REVIEW

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Application Reference

Laura Riva et al. J Virol 2021; 95 (22):e0099621 The Compound SBI-0090799 Inhibits Zika Virus Infection by Blocking De Novo Formation of the Membranous Replication Compartment.

Application : WB

Sarah Goellner et al. STAR Protoc 2020; 1 (3):100173 Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.

Application : WB, ICC/IF

Cerikan B et al. Cell Rep 2020; 32 (1):107859 A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.

Application : ICC/IF

Wang Y et al. Viruses 2020; 12 (5) TRiC/CCT Complex, a Binding Partner of NS1 Protein, Supports the Replication of Zika Virus in Both Mammalians and Mosquitoes.

Application : ICC/IF

SDS
PBS.pdf

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty

Zika virus NS1 protein antibody [GT5212]

Cat. No. GTX634158

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Research Area

DATA IMAGES

GTX634158 ICC/IF Image

GTX634158 ELISA Image

GTX634158 ICC/IF Image

GTX634158 ELISA Image

GTX634158 ELISA Image

GTX634158 WB Image

GTX634158 WB Image

GTX634158 ELISA Image

GTX634158 ELISA Image

GTX634158 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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