GeneTex
United States (US)

Zika virus NS1 protein antibody

GTX133307_42508_20160617_WB_Zika_2_18121410_277.jpg
GTX133307_42508_20160617_WB_Zika_18121410_327.jpg
GTX133307_42508_20160617_IFA_Zika_18121410_649.jpg
GTX133307_42508_20160617_IFA_Zika_2_18121410_740.jpg
GTX133307_42508_20160617_WB_Zika_3_18121410_827.jpg
GTX133307_42508_20170908_WB_B_Zika_18121410_797.jpg
GTX133307_42508_20181116_ELISA_PAIR_B_19011519_155.jpg

Cat No. GTX133307

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Zika virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:10000
ICC/IF 1:100-1:1000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Zika virus NS1-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Zika virus NS1 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zika virus NS1 protein antibody , Zika virus NS1 protein antibody

Research Area

DATA IMAGES
GTX133307_42508_20160617_WB_Zika_2_18121410_277.jpg

GTX133307 WB Image

Non-infected (–) and infected (+) C6/36 cells (20 µg) were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

GTX133307_42508_20160617_WB_Zika_18121410_327.jpg

GTX133307 WB Image

Non-infected (–) and infected (+) vero cells (15 µg) were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

GTX133307_42508_20160617_IFA_Zika_18121410_649.jpg

GTX133307 ICC/IF Image

Immunofluorescent analysis of Zika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus NS1 protein antibody (GTX133307).
Green: Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

GTX133307_42508_20160617_IFA_Zika_2_18121410_740.jpg

GTX133307 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero or BHK-21 cells using Zika virus NS1 protein antibody (GTX133307).
Green: Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

GTX133307_42508_20160617_WB_Zika_3_18121410_827.jpg

GTX133307 WB Image

Mock and infected Vero and BHK-21 whole cell extracts (20 µg) were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

GTX133307_42508_20170908_WB_B_Zika_18121410_797.jpg

GTX133307 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133307_42508_20181116_ELISA_PAIR_B_19011519_155.jpg

GTX133307 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 µg/mL and GTX133307 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE
REVIEW

Zika virus NS1 protein antibody Cat No. GTX133307

Rating ( Average 4.8 based on 5 users reviews)
Application
Western Blot(WB) ( Average 4.7 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, overnightHr
Date : Anonymous submitted on 13-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 3% BSA in PBS
Primary Antibody : 1:4000, 4°C, overnightHr
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, overnightHr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, overnightHr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 359
$ 169