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Zika virus NS1 protein antibody

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307
Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in ELISA (ELISA). GTX133307
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

Cat. No. GTX133307

Reference 36
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS1 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:10000
ICC/IF 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634158, Detection: GTX133307
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Zika virus NS1-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Zika virus NS1 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2688020

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 1 , Zika virus NS1 , ZIKV Non-structural protein 1 , ZIKV NS1

Research Area

DATA IMAGES

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

Non-infected (–) and infected (+) vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307

GTX133307 ICC/IF Image

Immunofluorescent analysis of Zika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus NS1 protein antibody (GTX133307).
Green: Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307

GTX133307 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero or BHK-21 cells using Zika virus NS1 protein antibody (GTX133307).
Green: Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody used in ELISA (ELISA). GTX133307

GTX133307 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133307 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

Mock and infected Vero and BHK-21 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

The data was published in the journal Front Microbiol in 2019. PMID: 31620112

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

The data was published in the 2019 in Sci Rep. PMID: 31534148

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

The data was published in the 2019 in Sci Rep. PMID: 31534148

REFERENCE

Application Reference

Mazar J et al. Cancer Res Commun 2024; 4 (1) : 65-80 The Oncolytic Activity of Zika Viral Therapy in Human Neuroblastoma In Vivo Models Confers a Major Survival Advantage in a CD24-dependent Manner.
Sahoo BR et al. J Virol 2023; 97 (2) : e0136322 Redox Regulation and Metabolic Dependency of Zika Virus Replication: Inhibition by Nrf2-Antioxidant Response and NAD(H) Antimetabolites.
Sawadpongpan S et al. BMC Complement Med Ther 2023; 23 (1) : 143 Investigation of the activity of baicalein towards Zika virus.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Hu D et al. Viruses 2023; 15 (1) ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein.
Application : WB
Show More +
Submit a Reference

REVIEW

Zika virus NS1 protein antibody Cat. No. GTX133307

Rating
( Average 4.8 based on 5 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307
Date : Anonymous submitted on 13-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 3% BSA in PBS
Primary Antibody : 1:4000, 4°C, 16Hr
Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, 16Hr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Submit a Review
Application Reference
Mazar J et al. Cancer Res Commun 2024; 4 (1):65-80 The Oncolytic Activity of Zika Viral Therapy in Human Neuroblastoma In Vivo Models Confers a Major Survival Advantage in a CD24-dependent Manner.
Sahoo BR et al. J Virol 2023; 97 (2):e0136322 Redox Regulation and Metabolic Dependency of Zika Virus Replication: Inhibition by Nrf2-Antioxidant Response and NAD(H) Antimetabolites.
Sawadpongpan S et al. BMC Complement Med Ther 2023; 23 (1):143 Investigation of the activity of baicalein towards Zika virus.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Hu D et al. Viruses 2023; 15 (1) ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein.
Application : WB
Rangel MV et al. J Virol 2022; 96 (2):e0177421 Structurally Conserved Domains between Flavivirus and Alphavirus Fusion Glycoproteins Contribute to Replication and Infectious-Virion Production.
Li S et al. Nat Commun 2022; 13 (1):105 Gain-of-function genetic screening identifies the antiviral function of TMEM120A via STING activation.
Application : WB
Danielle E Anderson et al. Proc Natl Acad Sci U S A 2021; 118 (39) Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.
Application : WB
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12):3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Zhang Y et al. PLoS Pathog 2021; 17 (8):e1009790 Efficient Dicer processing of virus-derived double-stranded RNAs and its modulation by RIG-I-like receptor LGP2.
Application : WB
Magalh?es ICL et al. Int J Biol Macromol 2021; 186:984-993 Non-structural protein 1 from Zika virus: Heterologous expression, purification, and potential for diagnosis of Zika infections.
Gao J et al. Virol J 2021; 18 (1):151 Construction of an infectious clone of Zika virus stably expressing an EGFP marker in a eukaryotic expression system.
Application : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
Application : WB
Xu P et al. PLoS Negl Trop Dis 2021; 15 (3):e0009183 Inhibition of innate immune response ameliorates Zika virus-induced neurogenesis deficit in human neural stem cells.
Application : WB
Khongwichit S et al. Sci Rep 2021; 11 (1):393 A functional interaction between GRP78 and Zika virus E protein.
Application : WB
Chen J et al. mBio 2020; 11 (5) Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
Srivastava M et al. Nat Commun 2020; 11 (1):3896 Chemical proteomics tracks virus entry and uncovers NCAM1 as Zika virus receptor.
Application : WB
Glover K et al. Viruses 2020; 12 (7) ZIKV Infection Induces DNA Damage Response and Alters the Proteome of Gastrointestinal Cells.
Application : WB
Karuppan MKM et al. Viruses 2020; 12 (6) Reduced-Beclin1-Expressing Mice Infected with Zika-R103451 and Viral-Associated Pathology during Pregnancy.
Application : ICC/IF
Wang Y et al. Viruses 2020; 12 (5) TRiC/CCT Complex, a Binding Partner of NS1 Protein, Supports the Replication of Zika Virus in Both Mammalians and Mosquitoes.
Application : WB
Danielle E Anderson et al. bioRxiv 2020; (Epub) Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy.
Application : WB
Liao X et al. Viruses 2020; 12 (4) 2', 5'-Oligoadenylate Synthetase 2 (OAS2) Inhibits Zika Virus Replication through Activation of Type ? IFN Signaling Pathway.
Application : WB
Hui L et al. PLoS Pathog 2020; 16 (4):e1008509 Matrix metalloproteinase 9 facilitates Zika virus invasion of the testis by modulating the integrity of the blood-testis barrier.
Garcia G Jr et al. Am J Pathol 2020; (Epub) Hippo Signaling Pathway Has a Critical Role in Zika Virus Replication and in the Pathogenesis of Neuroinflammation.
Application : WB
Grubor-Bauk B et al. Sci Adv 2019; 5 (12):eaax2388 NS1 DNA vaccination protects against Zika infection through T cell-mediated immunity in immunocompetent mice.
Lu CY et al. Viruses 2019; 11 (11) The Rescue and Characterization of Recombinant, Microcephaly-Associated Zika Viruses as Single-Round Infectious Particles.
Application : ICC/IF
Leda AR et al. Front Microbiol 2019; 10:2158 Selective Disruption of the Blood-Brain Barrier by Zika Virus.
Application : WB, ICC/IF
Zhang X et al. MBio 2019; 10 (5) Zika Virus NS2A-Mediated Virion Assembly.
Application : WB
Sornjai W et al. Sci Rep 2019; 9 (1):13498 High correlation between Zika virus NS1 antibodies and neutralizing antibodies in selected serum samples from normal healthy Thais.
Application : WB
Annamalai AS et al. Vaccines (Basel) 2019; 7 (3) An Attenuated Zika Virus Encoding Non-Glycosylated Envelope (E) and Non-Structural Protein 1 (NS1) Confers Complete Protection against Lethal Challenge in a Mouse Model.
Application : WB
Takemura K et al. PLoS One 2019; 14 (1):e0211517 A localized surface plasmon resonance-amplified immunofluorescence biosensor for ultrasensitive and rapid detection of nonstructural protein 1 of Zika virus.
Li P et al. Cell Host Microbe 2018; 24 (6):875-886.e5 Integrative Analysis of Zika Virus Genome RNA Structure Reveals Critical Determinants of Viral Infectivity.
Application : WB
Chen J et al. Nat Microbiol 2018; 3 (3):302-309 axl promotes zika virus infection in astrocytes by antagonizing type i * signalling.
Application : WB
Khaiboullina SF et al. Sci Rep 2017; 7 (1):16050 ZIKV infection regulates inflammasomes pathway for replication in monocytes.
Application : WB, ICC/IF
Yoon KJ et al. Cell Stem Cell 2017; 21 (3):349-358.e6 Zika-Virus-Encoded NS2A Disrupts Mammalian Cortical Neurogenesis by Degrading Adherens Junction Proteins.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169