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Zika virus NS1 protein antibody

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307
Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in ELISA (ELISA). GTX133307

Cat. No. GTX133307

Reference 34
8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS1 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:10000
ICC/IF 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634158, Detection: GTX133307
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Zika virus NS1-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Zika virus NS1 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2688020

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Non-structural protein 1 , Zika virus NS1 , ZIKV Non-structural protein 1 , ZIKV NS1

Research Area

DATA IMAGES

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

Non-infected (–) and infected (+) vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307

GTX133307 ICC/IF Image

Immunofluorescent analysis of Zika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus NS1 protein antibody (GTX133307).
Green: Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307

GTX133307 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero or BHK-21 cells using Zika virus NS1 protein antibody (GTX133307).
Green: Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

Mock and infected Vero and BHK-21 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody used in ELISA (ELISA). GTX133307

GTX133307 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133307 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

Application Reference

Sawadpongpan S et al. BMC Complement Med Ther 2023; 23 (1) : 143 Investigation of the activity of baicalein towards Zika virus.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Hu D et al. Viruses 2023; 15 (1) ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein.
Rangel MV et al. J Virol 2022; 96 (2) : e0177421 Structurally Conserved Domains between Flavivirus and Alphavirus Fusion Glycoproteins Contribute to Replication and Infectious-Virion Production.
Li S et al. Nat Commun 2022; 13 (1) : 105 Gain-of-function genetic screening identifies the antiviral function of TMEM120A via STING activation.
Application : WB
Show More +
Submit a Reference

REVIEW

Zika virus NS1 protein antibody Cat. No. GTX133307

Rating
( Average 4.9 based on 8 users reviews)
Application
Western Blot(WB)
( Average 4.8 based on 6 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2019 in Sci Rep. PMID: 31534148
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2019 in Sci Rep. PMID: 31534148
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2019. PMID: 31620112
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307
Date : Anonymous submitted on 13-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 3% BSA in PBS
Primary Antibody : 1:4000, 4°C, 16Hr
Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, 16Hr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Show More +
Submit a Review
Application Reference
Sawadpongpan S et al. BMC Complement Med Ther 2023; 23 (1):143 Investigation of the activity of baicalein towards Zika virus.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Hu D et al. Viruses 2023; 15 (1) ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein.
Rangel MV et al. J Virol 2022; 96 (2):e0177421 Structurally Conserved Domains between Flavivirus and Alphavirus Fusion Glycoproteins Contribute to Replication and Infectious-Virion Production.
Li S et al. Nat Commun 2022; 13 (1):105 Gain-of-function genetic screening identifies the antiviral function of TMEM120A via STING activation.
Application : WB
Danielle E Anderson et al. Proc Natl Acad Sci U S A 2021; 118 (39) Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.
Application : WB
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12):3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Zhang Y et al. PLoS Pathog 2021; 17 (8):e1009790 Efficient Dicer processing of virus-derived double-stranded RNAs and its modulation by RIG-I-like receptor LGP2.
Application : WB
Magalh?es ICL et al. Int J Biol Macromol 2021; 186:984-993 Non-structural protein 1 from Zika virus: Heterologous expression, purification, and potential for diagnosis of Zika infections.
Gao J et al. Virol J 2021; 18 (1):151 Construction of an infectious clone of Zika virus stably expressing an EGFP marker in a eukaryotic expression system.
Application : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
Application : WB
Xu P et al. PLoS Negl Trop Dis 2021; 15 (3):e0009183 Inhibition of innate immune response ameliorates Zika virus-induced neurogenesis deficit in human neural stem cells.
Application : WB
Khongwichit S et al. Sci Rep 2021; 11 (1):393 A functional interaction between GRP78 and Zika virus E protein.
Application : WB
Chen J et al. mBio 2020; 11 (5) Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
Srivastava M et al. Nat Commun 2020; 11 (1):3896 Chemical proteomics tracks virus entry and uncovers NCAM1 as Zika virus receptor.
Application : WB
Glover K et al. Viruses 2020; 12 (7) ZIKV Infection Induces DNA Damage Response and Alters the Proteome of Gastrointestinal Cells.
Application : WB
Karuppan MKM et al. Viruses 2020; 12 (6) Reduced-Beclin1-Expressing Mice Infected with Zika-R103451 and Viral-Associated Pathology during Pregnancy.
Application : ICC/IF
Wang Y et al. Viruses 2020; 12 (5) TRiC/CCT Complex, a Binding Partner of NS1 Protein, Supports the Replication of Zika Virus in Both Mammalians and Mosquitoes.
Application : WB
Danielle E Anderson et al. bioRxiv 2020; (Epub) Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy.
Application : WB
Liao X et al. Viruses 2020; 12 (4) 2', 5'-Oligoadenylate Synthetase 2 (OAS2) Inhibits Zika Virus Replication through Activation of Type ? IFN Signaling Pathway.
Application : WB
Hui L et al. PLoS Pathog 2020; 16 (4):e1008509 Matrix metalloproteinase 9 facilitates Zika virus invasion of the testis by modulating the integrity of the blood-testis barrier.
Garcia G Jr et al. Am J Pathol 2020; (Epub) Hippo Signaling Pathway Has a Critical Role in Zika Virus Replication and in the Pathogenesis of Neuroinflammation.
Application : WB
Grubor-Bauk B et al. Sci Adv 2019; 5 (12):eaax2388 NS1 DNA vaccination protects against Zika infection through T cell-mediated immunity in immunocompetent mice.
Lu CY et al. Viruses 2019; 11 (11) The Rescue and Characterization of Recombinant, Microcephaly-Associated Zika Viruses as Single-Round Infectious Particles.
Application : ICC/IF
Leda AR et al. Front Microbiol 2019; 10:2158 Selective Disruption of the Blood-Brain Barrier by Zika Virus.
Application : WB, ICC/IF
Zhang X et al. MBio 2019; 10 (5) Zika Virus NS2A-Mediated Virion Assembly.
Application : WB
Sornjai W et al. Sci Rep 2019; 9 (1):13498 High correlation between Zika virus NS1 antibodies and neutralizing antibodies in selected serum samples from normal healthy Thais.
Application : WB
Annamalai AS et al. Vaccines (Basel) 2019; 7 (3) An Attenuated Zika Virus Encoding Non-Glycosylated Envelope (E) and Non-Structural Protein 1 (NS1) Confers Complete Protection against Lethal Challenge in a Mouse Model.
Application : WB
Takemura K et al. PLoS One 2019; 14 (1):e0211517 A localized surface plasmon resonance-amplified immunofluorescence biosensor for ultrasensitive and rapid detection of nonstructural protein 1 of Zika virus.
Li P et al. Cell Host Microbe 2018; 24 (6):875-886.e5 Integrative Analysis of Zika Virus Genome RNA Structure Reveals Critical Determinants of Viral Infectivity.
Application : WB
Chen J et al. Nat Microbiol 2018; 3 (3):302-309 axl promotes zika virus infection in astrocytes by antagonizing type i * signalling.
Application : WB
Khaiboullina SF et al. Sci Rep 2017; 7 (1):16050 ZIKV infection regulates inflammasomes pathway for replication in monocytes.
Application : WB, ICC/IF
Yoon KJ et al. Cell Stem Cell 2017; 21 (3):349-358.e6 Zika-Virus-Encoded NS2A Disrupts Mammalian Cortical Neurogenesis by Degrading Adherens Junction Proteins.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169