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Zika virus NS1 protein antibody

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307
Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307
Anti-Zika virus NS1 protein antibody used in ELISA (ELISA). GTX133307

Cat No. GTX133307

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

Zika virus
Package
100 μl ($359),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:10000
ICC/IF 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634158, Detection: GTX133307

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Zika virus NS1-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Zika virus NS1 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2688020

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Non-structural protein 1 , Zika virus NS1 , ZIKV Non-structural protein 1 , ZIKV NS1

Research Area

DATA IMAGES

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

Non-infected (–) and infected (+) vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307

GTX133307 ICC/IF Image

Immunofluorescent analysis of Zika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus NS1 protein antibody (GTX133307).
Green: Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133307

GTX133307 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero or BHK-21 cells using Zika virus NS1 protein antibody (GTX133307).
Green: Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

Mock and infected Vero and BHK-21 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133307

GTX133307 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133307) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody used in ELISA (ELISA). GTX133307

GTX133307 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133307 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

REVIEW

Zika virus NS1 protein antibody Cat No. GTX133307

Rating ( Average 4.8 based on 6 users reviews)
Application
Western Blot(WB) ( Average 4.8 based on 4 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2019.PMID: 31620112
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Date : Anonymous submitted on 13-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 3% BSA in PBS
Primary Antibody : 1:4000, 4°C, 16Hr
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, 16Hr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf