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Zika virus NS1 protein antibody

Anti-Zika virus NS1 protein antibody used in ELISA (ELISA). GTX133324
Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133324
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133324
Anti-Zika virus NS1 protein antibody used in ELISA (ELISA). GTX133324
Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133324

Cat. No. GTX133324

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS1 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634158 / GTX133324, Detection : GTX133324 / GTX634158
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Zika-infected Vero , Zika virus NS1-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Zika virus NS1 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886929

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 1 , Zika virus NS1 , ZIKV Non-structural protein 1 , ZIKV NS1

Research Area

DATA IMAGES

Anti-Zika virus NS1 protein antibody used in ELISA (ELISA). GTX133324

GTX133324 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133324 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133324

GTX133324 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus NS1 protein antibody (GTX133324).
Green: Zika virus NS1 protein antibody (GTX133324) diluted at 1:500.

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133324

GTX133324 WB Image

Non-infected (–) and infected (+) Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133324) diluted at 1:2500.

Anti-Zika virus NS1 protein antibody used in ELISA (ELISA). GTX133324

GTX133324 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX133324 as capture antibody at concentration of 5 μg/mL and GTX634158 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus NS1 protein antibody used in Western Blot (WB). GTX133324

GTX133324 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133324) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169