Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133324) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX133324 as capture antibody at concentration of 5 μg/mL and GTX634158 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Non-infected (–) and infected (+) Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody (GTX133324) diluted at 1:2500.

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus NS1 protein antibody (GTX133324).
Green: Zika virus NS1 protein antibody (GTX133324) diluted at 1:500.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 μg/mL and GTX133324 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.