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Zika virus NS2B protein antibody

Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308
Anti-Zika virus NS2B protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133308

Cat No. GTX133308

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Zika virus
Package
100 μl ($359),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus NS2B protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2715494

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Non-structural protein 2B , Zika virus NS2B , ZIKV Non-structural protein 2B , ZIKV NS2B

Research Area

DATA IMAGES

Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308

GTX133308 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS2B protein antibody (GTX133308).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS2B protein antibody (GTX133308) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308

GTX133308 WB Image

Non-infected (–) and infected (+) C6/36 cells (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS2B protein antibody (GTX133308) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308

GTX133308 WB Image

Non-infected (–) and infected (+) vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS2B protein antibody (GTX133308) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308

GTX133308 ICC/IF Image

Immunofluorescent analysis ofZika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus NS2B protein antibody (GTX133308).
Green: Zika virus NS2B protein antibody (GTX133308) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS2B protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133308

GTX133308 IHC-P Image

Zika virus NS2B protein antibody detects Zika virus NS2B protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded zika virus C6/36.
Green: Zika virus NS2B protein stained by Zika virus NS2B protein antibody (GTX133308) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Zika virus NS2B protein antibody Cat No. GTX133308

Rating ( Average 5 based on 10 users reviews)
Application
Western Blot(WB) ( Average 5 based on 7 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Elife in 2018.PMID: 30511641
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Negl Trop Dis in 2017.PMID: 28481898
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected Vero cells
Amount used : 20 μg
Blocking : 5% non-fat milk, 22°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : The researcher provided "Cpd-E2 is a novel antiviral affecting Zika polyprotein translation".
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected Huh-7 cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected C6/36 cells, Zika infected vero cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 08-Sep-2016
Score :
Application Tested : IHC-P
Sample Species : ZIKV
Sample : Zika-infected mouse spleen tissues
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : A: mouse red pulp tissue, B: Zika virus infected mouse red pulp tissue
Date : Anonymous submitted on 08-Sep-2016
Score :
Application Tested : IHC-P
Sample Species : ZIKV
Sample : Zika-infected mouse spleen tissues
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : A: mouse white pulp tissue, B: Zika virus infected mouse white pulp tissue
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, 16Hr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf