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Zika virus NS2B protein antibody

Orthogonal
Anti-Zika virus NS2B protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133308
Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308

Cat. No. GTX133308

Reference 33
10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-P (cell pellet)

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS2B protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus NS2B protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2715494

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Non-structural protein 2B , Zika virus NS2B , ZIKV Non-structural protein 2B , ZIKV NS2B

Research Area

DATA IMAGES

Anti-Zika virus NS2B protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133308

GTX133308 IHC-P (cell pellet) Image

Zika virus NS2B protein antibody detects Zika virus NS2B protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded zika virus C6/36.
Green: Zika virus NS2B protein stained by Zika virus NS2B protein antibody (GTX133308) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308

GTX133308 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS2B protein antibody (GTX133308).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS2B protein antibody (GTX133308) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308

GTX133308 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus NS2B protein antibody (GTX133308) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308

GTX133308 ICC/IF Image

Immunofluorescent analysis ofZika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus NS2B protein antibody (GTX133308).
Green: Zika virus NS2B protein antibody (GTX133308) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

REFERENCE

Application Reference

Zhuang Wang et al. Viruses 2022; 14 (6) Azelnidipine Exhibits In Vitro and In Vivo Antiviral Effects against Flavivirus Infections by Targeting the Viral RdRp.
Kung YA et al. mBio 2022; : e0271721 Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.
Application : ICC/IF
Jose Carlos Ponce-Rojas et al. J Clin Microbiol 2021; 59 (4) A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein To Facilitate the Detection of SARS-CoV-2.
Application : WB
Ferreira RO et al. Viruses 2021; 13 (10) Effect of Serial Systemic and Intratumoral Injections of Oncolytic ZIKVBR in Mice Bearing Embryonal CNS Tumors.
Application : IHC-Fr
Gavino-Leopoldino D et al. J Virol 2021; 95 (22) : e0090421 Skeletal Muscle Is an Early Site of Zika Virus Replication and Injury, Which Impairs Myogenesis.
Application : ICC/IF
Show More +
Submit a Reference

REVIEW

Zika virus NS2B protein antibody Cat. No. GTX133308

Rating
( Average 5 based on 10 users reviews)
Application
Western Blot(WB)
( Average 5 based on 7 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Elife in 2018.PMID: 30511641
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Negl Trop Dis in 2017.PMID: 28481898
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected Vero cells
Amount used : 20 μg
Blocking : 5% non-fat milk, 22°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : The researcher provided "Cpd-E2 is a novel antiviral affecting Zika polyprotein translation".
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected Huh-7 cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected C6/36 cells, Zika infected vero cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-Zika virus NS2B protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133308
Date : Anonymous submitted on 08-Sep-2016
Score :
Application Tested : IHC-P
Sample Species : ZIKV
Sample : Zika-infected mouse spleen tissues
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : A: mouse red pulp tissue, B: Zika virus infected mouse red pulp tissue
Anti-Zika virus NS2B protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133308
Date : Anonymous submitted on 08-Sep-2016
Score :
Application Tested : IHC-P
Sample Species : ZIKV
Sample : Zika-infected mouse spleen tissues
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : A: mouse white pulp tissue, B: Zika virus infected mouse white pulp tissue
Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, 16Hr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Show More +
Submit a Review
Application Reference
Zhuang Wang et al. Viruses 2022; 14 (6) Azelnidipine Exhibits In Vitro and In Vivo Antiviral Effects against Flavivirus Infections by Targeting the Viral RdRp.
Kung YA et al. mBio 2022;:e0271721 Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.
Application : ICC/IF
Jose Carlos Ponce-Rojas et al. J Clin Microbiol 2021; 59 (4) A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein To Facilitate the Detection of SARS-CoV-2.
Application : WB
Ferreira RO et al. Viruses 2021; 13 (10) Effect of Serial Systemic and Intratumoral Injections of Oncolytic ZIKVBR in Mice Bearing Embryonal CNS Tumors.
Application : IHC-Fr
Gavino-Leopoldino D et al. J Virol 2021; 95 (22):e0090421 Skeletal Muscle Is an Early Site of Zika Virus Replication and Injury, Which Impairs Myogenesis.
Application : ICC/IF
Shelton SM et al. eNeuro 2021; 8 (5) Forebrain Neural Precursor Cells Are Differentially Vulnerable to Zika Virus Infection.
Application : IHC-Fr
Matz K et al. Microorganisms 2021; 9 (6) Favipiravir (T-705) Protects IFNAR-/- Mice against Lethal Zika Virus Infection in a Sex-Dependent Manner.
Application : IHC-P
Pletnev AG et al. Nat Commun 2021; 12 (1):2469 Epididymal epithelium propels early sexual transmission of Zika virus in the absence of interferon signaling.
Silva BVM et al. Biosensors (Basel) 2021; 11 (5) A Label and Probe-Free Zika Virus Immunosensor Prussian Blue@carbon Nanotube-Based for Amperometric Detection of the NS2B Protein.
Shi H et al. Biotechnol Bioeng 2021; (Epub) Kinetics of Asian and African Zika Virus Lineages over Single-cycle and Multi-cycle Growth in Culture: gene expression, cell killing, virus production, and mathematical modeling.
Rory K M Long et al. Sci Rep 2020; 10 (1):20937 Super resolution microscopy and deep learning identify Zika virus reorganization of the endoplasmic reticulum.
Application : ICC/IF
Kaid C et al. Mol Ther 2020; 28 (5):1276-1286 Safety, Tumor Reduction, and Clinical Impact of Zika Virus Injection in Dogs with Advanced-Stage Brain Tumors.
Xiangling Cui et al. Front Pharmacol. 2020; 11:514313 Identification of Theaflavin-3,3'-Digallate as a Novel Zika Virus Protease Inhibitor.
Application : WB
Chen J et al. mBio 2020; 11 (5) Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
Application : ICC/IF
Tsetsarkin KA et al. PLoS Pathog 2020; 16 (7):e1008601 Zika virus tropism during early infection?of the?testicular interstitium and its role in viral pathogenesis in the testes.
Application : IHC-P
Wu Y et al. PLoS Negl Trop Dis 2020; 14 (3):e0008083 C19orf66 interrupts Zika virus replication by inducing lysosomal degradation of viral NS3.
Application : WB
Zhang X et al. MBio 2019; 10 (5) Zika Virus NS2A-Mediated Virion Assembly.
Application : WB
Figueiredo CP et al. Nat Commun 2019; 10 (1):3890 Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice.
Application : IHC-P, IHC-Fr
Barrows NJ et al. Sci Rep 2019; 9 (1):9711 Dual roles for the ER membrane protein complex in flavivirus infection: viral entry and protein biogenesis.
Application : WB
Branche E et al. Sci Rep 2019; 9 (1):9857 Human Polyclonal Antibodies Prevent Lethal Zika Virus Infection in Mice.
Application : ICC/IF, IHC-P
Regla-Nava JA et al. PLoS Negl Trop Dis 2019; 13 (2):e0007080 Detection of Zika virus in mouse mammary gland and breast milk.
Application : ICC/IF, IHC-P
Carlin AF et al. Proc Natl Acad Sci U S A 2018; 115 (39):E9172-E9181 Deconvolution of pro- and antiviral genomic responses in Zika virus-infected and bystander macrophages.
Tsetsarkin KA et al. Nat Commun 2018; 9 (1):5350 Routes of Zika virus dissemination in the testis and epididymis of immunodeficient mice.
Soto-Acosta R et al. Elife 2018; 7 Fragile X mental retardation protein is a Zika virus restriction factor that is antagonized by subgenomic flaviviral RNA.
Application : WB
Wu YH et al. PLoS Negl Trop Dis 2018; 12 (10):e0006848 ICR suckling mouse model of Zika virus infection for disease modeling and drug validation.
Application : WB
Chen J et al. Nat Microbiol 2018; 3 (3):302-309 axl promotes zika virus infection in astrocytes by antagonizing type i * signalling.
Application : WB
Fernandez MP et al. JAMA Ophthalmol 2017; 135 (11):1163-1169 Ocular Histopathologic Features of Congenital Zika Syndrome.
Boigard H et al. PLoS Negl Trop Dis 2017; 11 (5):e0005608 Zika virus-like particle (VLP) based vaccine.
Fernandez M.P. et al. JAMA Ophthalmology 2017; (Epub) Ocular Histopathologic Features of Congenital Zika Syndrome.
Application : IHC-P
Bollweg BC et al. The Journal of Pathology 2017; (Epub) Optimization of Commercially Available Zika Virus Antibodies for Use in a Laboratory Developed Immunohistochemical Assay.
Application : IHC-P
Nguyen SM et al. PLoS Pathog 2017; 13 (5):e1006378 Highly efficient maternal-fetal Zika virus transmission in pregnant rhesus macaques.
Application : IHC-P
Boigard H et al. PLoS Negl Trop Dis 2017; (Epub) Zika virus-like particle (VLP) based vaccine.
Application : WB
Tang WW et al. Cell Reports 2016; (Epub) A Mouse Model of Zika Virus Sexual Transmission and Vaginal Viral Replication.
Application : IHC-P
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169