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Zika virus NS2B protein antibody

Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308
Anti-Zika virus NS2B protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133308

Cat No. GTX133308

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Zika virus
Package
100 μl ($359),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.29 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus NS2B protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zika virus NS2B protein antibody , Zika virus NS2B protein antibody

Research Area

DATA IMAGES
Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308

GTX133308 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS2B protein antibody (GTX133308).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS2B protein antibody (GTX133308) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308

GTX133308 WB Image

Non-infected (–) and infected (+) C6/36 cells (20 µg) were separated by gradient gel, and the membrane was blotted with Zika virus NS2B protein antibody (GTX133308) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308

GTX133308 WB Image

Non-infected (–) and infected (+) vero cells (15 µg) were separated by gradient gel, and the membrane was blotted with Zika virus NS2B protein antibody (GTX133308) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308

GTX133308 ICC/IF Image

Immunofluorescent analysis ofZika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus NS2B protein antibody (GTX133308).
Green: Zika virus NS2B protein antibody (GTX133308) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS2B protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133308

GTX133308 IHC-P Image

Zika virus NS2B protein antibody detects Zika virus NS2B protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded zika virus C6/36.
Green: Zika virus NS2B protein stained by Zika virus NS2B protein antibody (GTX133308) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

Zika virus NS2B protein antibody Cat No. GTX133308

Rating ( Average 5 based on 8 users reviews)
Application
Western Blot(WB) ( Average 5 based on 5 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected Vero cells
Amount used : 20 μg
Blocking : 5% non-fat milk, 22°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : The researcher provided "Cpd-E2 is a novel antiviral affecting Zika polyprotein translation". Image courtesy of Darrell Davis Laboratory, University of Utah.
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected Huh-7 cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, overnightHr
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected C6/36 cells, Zika infected vero cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, overnightHr
Date : Anonymous submitted on 08-Sep-2016
Score :
Application Tested : IHC-P
Sample Species : ZIKV
Sample : Zika-infected mouse spleen tissues
Primary Antibody : 1:100, 4°C, overnightHr
Figure Note : A: mouse red pulp tissue, B: Zika virus infected mouse red pulp tissue
Date : Anonymous submitted on 08-Sep-2016
Score :
Application Tested : IHC-P
Sample Species : ZIKV
Sample : Zika-infected mouse spleen tissues
Primary Antibody : 1:100, 4°C, overnightHr
Figure Note : A: mouse white pulp tissue, B: Zika virus infected mouse white pulp tissue
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, overnightHr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, overnightHr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 359
$ 169