Zika virus NS2B protein antibody

Name: Zika virus NS2B protein antibody
Brand: GeneTex
SKU: GTX133318
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133318

Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133318

Cat. No. GTX133318

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Zika virus
Package	100 μl ($399), 25 μl ($169)

See all Zika virus NS2B protein products

View product citations for antibody GTX133318 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:20000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Zika virus-infected Vero , Zika virus NS2B-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Full length Zika virus NS2B recombinant protein. (Zika virus (strain H/PF/2013))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886925

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 2B , Zika virus NS2B , ZIKV Non-structural protein 2B , ZIKV NS2B

Research Area

DATA IMAGES

GTX133318 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus NS2B protein antibody (GTX133318) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133318 WB Image

Non-infected (–) and infected (+) Vero whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS2B protein antibody (GTX133318) diluted at 1:15000.

GTX133318 ICC/IF Image

Zika virus NS2B protein antibody detects Zika virus NS2B protein at cytoplasm by immunofluorescent analysis.
Sample: Vero cells were fixed in 4% paraformaldehyde at RT for 30 min.
Green: Zika virus NS2B stained by Zika virus NS2B protein antibody (GTX133318) diluted at 1:1000.
Blue: nuclear staining.

REFERENCE

Application Reference

Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.

Application : WB

Sarah Goellner et al. STAR Protoc 2020; 1 (3) : 100173 Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.

Application : WB, ICC/IF

Cerikan B et al. Cell Rep 2020; 32 (1) : 107859 A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.

Application : ICC/IF

Jia He et al. Autophagy 2020; (Epub) Zika virus NS2A protein induces the degradation of KPNA2 (karyopherin subunit alpha 2) via chaperone-mediated autophagy.

Application : WB

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