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Zika virus NS2B protein antibody

Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133318
Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133318
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133318

Cat No. GTX133318

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Zika virus
Package
100 μl ($359),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Zika NS2B-transfected 293T , Zika virus-infected Vero
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Full length Zika virus NS2B recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zika virus NS2B antibody

Research Area

DATA IMAGES
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133318

GTX133318 WB Image

Non-infected (–) and infected (+) Vero whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS2B protein antibody (GTX133318) diluted at 1:15000.

Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133318

GTX133318 ICC/IF Image

Zika virus NS2B protein antibody detects Zika virus NS2B protein at cytoplasm by immunofluorescent analysis.
Sample: Vero cells were fixed in 4% paraformaldehyde at RT for 30 min.
Green: Zika virus NS2B stained by Zika virus NS2B protein antibody (GTX133318) diluted at 1:1000.
Blue: nuclear staining.

Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133318

GTX133318 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus NS2B protein antibody (GTX133318) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW

Zika virus NS2B protein antibody Cat No. GTX133318

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 31-Mar-2018
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Blocking : milk, 1Hr
Fixation : 4% Paraformaldehyde, 0.5Hr
Permeabilization /Antigen retrieval : 0.5% Triton X-100, 10 minHr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Zika Virus-PRVABC59 infected vero cells (MOI=5 24h)
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 359
$ 169