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Zika virus NS3 protein antibody

Orthogonal
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133309

Cat No. GTX133309

Reference 43
8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Zika virus
Package
100 μl ($379),
25 μl ($169)
See all Zika virus NS3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Zika virus NS3 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2756864

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Non-structural protein 3 , Zika virus NS3 , ZIKV Non-structural protein 3 , ZIKV NS3

Research Area

DATA IMAGES

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

Mock and infected Vero and BHK-21 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

Non-infected (–) and infected (+) vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

Non-infected (–) and infected (+) C6/36 cells (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133309

GTX133309 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS3 protein antibody (GTX133309).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS3 protein antibody (GTX133309) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Mahamud-Ur Rashid et al. Viruses 2022; 14 (2) Zika Virus Infection of Sertoli Cells Alters Protein Expression Involved in Activated Immune and Antiviral Response Pathways, Carbohydrate Metabolism and Cardiovascular Disease.
Kung YA et al. mBio 2022; : e0271721 Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.
Application : ICC/IF
Xi Yu et al. Proc Natl Acad Sci U S A 2021; 118 (42) A mutation-mediated evolutionary adaptation of Zika virus in mosquito and mammalian host.
Weiqiang Chen et al. Nat Microbiol 2021; 6 (4) : 455-466 Zika virus NS3 protease induces bone morphogenetic protein-dependent brain calcification in human fetuses.
Jun Shu et al. Emerg Microbes Infect 2021; 10 (1) : 1609-1625 NS5-independent Ablation of STAT2 by Zika virus to antagonize interferon signalling.
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Submit a Reference

REVIEW

Zika virus NS3 protein antibody Cat No. GTX133309

Rating
( Average 5 based on 8 users reviews)
Application
Western Blot(WB)
( Average 5 based on 8 users reviews)
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 22-Feb-2022
Score :
Application Tested : WB
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the PLoS Pathog in 2018. PMID: 30142214
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2017. PMID: 28588451
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2020. PMID: 32277605
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017. PMID: 28542603
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2019. PMID: 30984137
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 03-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Show More +
Submit a Review
Application Reference
Mahamud-Ur Rashid et al. Viruses 2022; 14 (2) Zika Virus Infection of Sertoli Cells Alters Protein Expression Involved in Activated Immune and Antiviral Response Pathways, Carbohydrate Metabolism and Cardiovascular Disease.
Kung YA et al. mBio 2022;:e0271721 Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.
Application : ICC/IF
Patthara Kongsuphol et al. Commun Med (Lond) 2021; 1:46 A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Xi Yu et al. Proc Natl Acad Sci U S A 2021; 118 (42) A mutation-mediated evolutionary adaptation of Zika virus in mosquito and mammalian host.
Weiqiang Chen et al. Nat Microbiol 2021; 6 (4):455-466 Zika virus NS3 protease induces bone morphogenetic protein-dependent brain calcification in human fetuses.
Jun Shu et al. Emerg Microbes Infect 2021; 10 (1):1609-1625 NS5-independent Ablation of STAT2 by Zika virus to antagonize interferon signalling.
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12):3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Kongsuphol P. et al. Communications Medicine 2021; (Epub) A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Jhan MK et al. Cells 2021; 10 (11) Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.
Application : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
Application : WB
Gambino F Jr et al. Cell Rep 2021; 35 (6):109107 A vaccine inducing solely cytotoxic T lymphocytes fully prevents Zika virus infection and fetal damage.
Application : WB
Yefeng Ji et al. Cancer Med 2020; 9 (11):3954-3963 Long noncoding RNA MEG3 decreases the growth of head and neck squamous cell carcinoma by regulating the expression of miR-421 and E-cadherin.
Application : WB
Li Z et al. Emerg Microbes Infect 2020; 9 (1):2404-2416 Methylene blue is a potent and broad-spectrum inhibitor against Zika virus in vitro and in vivo.
Chen J et al. mBio 2020; 11 (5) Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
Yuan J et al. BMC Med 2020; 18 (1):204 Antibiotic fidaxomicin is an RdRp inhibitor as a potential new therapeutic agent against Zika virus.
Chen Q et al. Nat Commun 2020; 11 (1):2967 Metabolic reprogramming by Zika virus provokes inflammation in human placenta.
Clark AE et al. J Virol 2020; (Epub) Zika virus is transmitted in neural progenitor cells via cell-to-cell spread and infection is inhibited by the autophagy inducer trehalose.
Application : WB
Elong Ngono A et al. Sci Adv 2020; 6 (45) CD8+ T cells mediate protection against Zika virus induced by an NS3-based vaccine.
Application : WB
Li Z et al. ACS Infect Dis 2020; 6 (10):2616-2628 JMX0207, a Niclosamide Derivative with Improved Pharmacokinetics, Suppresses Zika Virus Infection Both In Vitro and In Vivo.
Application : WB
Yuan S et al. Sci Adv 2020; 6 (35):eaba7910 Viruses harness Yxx? motif to interact with host AP2M1 for replication: A vulnerable broad-spectrum antiviral target.
Application : IHC-P
De Jes?s-Gonz?lez LA et al. Viruses 2020; 12 (6) The Nuclear Pore Complex: A Target for NS3 Protease of Dengue and Zika Viruses.
Application : WB
Guang Song et al. bioRxiv 2020; (Epub) An Integrated Systems Biology Approach Identifies the Proteasome as a Critical Host Machinery for ZIKV and DENV Replication.
Application : WB
Wu Y et al. PLoS Negl Trop Dis 2020; 14 (3):e0008083 C19orf66 interrupts Zika virus replication by inducing lysosomal degradation of viral NS3.
Application : WB
Wen F et al. J Biol Chem 2019; 294 (49):18742-18755 Zika virus increases mind bomb 1 levels, causing degradation of pericentriolar material 1 (PCM1) and dispersion of PCM1-containing granules from the centrosome.
Whelan JN et al. J Virol 2019; 93 (16) Zika Virus Production Is Resistant to RNase L Antiviral Activity.
Zhang X et al. MBio 2019; 10 (5) Zika Virus NS2A-Mediated Virion Assembly.
Application : WB
Nandan S. Gokhale et al. bioRxiv 2019; (Epub) Altered m6A modification of specific cellular transcripts affects Flaviviridae infection
Application : WB
Li W et al. FASEB J 2019;:fj201900260R Zika virus circumvents host innate immunity by targeting the adaptor proteins MAVS and MITA.
Barrows NJ et al. Sci Rep 2019; 9 (1):9711 Dual roles for the ER membrane protein complex in flavivirus infection: viral entry and protein biogenesis.
Application : WB
Sher AA et al. Front Microbiol 2019; 10:596 Zika Virus Infection Disrupts Astrocytic Proteins Involved in Synapse Control and Axon Guidance.
Application : WB
Taguwa S et al. Cell Rep 2019; 26 (4):906-920.e3 Zika Virus Dependence on Host Hsp70 Provides a Protective Strategy against Infection and Disease.
Application : ICC/IF
Wang K et al. PLoS Pathog 2018; 14 (8):e1007287 *-stimulated trim69 interrupts dengue virus replication by ubiquitinating viral nonstructural protein 3.
Application : WB
Margot Cervantes-Salazar et al. bioRxiv 2018; (Epub) The Nonstructural Proteins 3 And 5 From Flavivirus Modulate Nuclear-Cytoplasmic Transport And Innate Immune Response Targeting Nuclear Proteins.
Application : WB
McFadden MJ et al. Viruses 2018; 10 (2) A Fluorescent Cell-Based System for Imaging Zika Virus Infection in Real-Time.
Application : WB
Chen J et al. Nat Microbiol 2018; 3 (3):302-309 axl promotes zika virus infection in astrocytes by antagonizing type i * signalling.
Application : WB
Robert Bronstein et al. Front Mol Neurosci 2017; 10 (0):153 Neurogenic to Gliogenic Fate Transition Perturbed by Loss of HMGB2.
Application : WB
Li Z et al. Cell Res 2017; 27 (8):1046-1064 Existing drugs as broad-spectrum and potent inhibitors for Zika virus by targeting NS2B-NS3 interaction.
Boigard H et al. PLoS Negl Trop Dis 2017; 11 (5):e0005608 Zika virus-like particle (VLP) based vaccine.
Li Z et al. Antiviral Res 2017; 150:217-225 Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease.
Application : WB
Brecher M et al. PLoS Pathog 2017; 13 (5):e1006411 A conformational switch high-throughput screening assay and allosteric inhibition of the flavivirus NS2B-NS3 protease.
Application : WB
Boigard H et al. PLoS Negl Trop Dis 2017; (Epub) Zika virus-like particle (VLP) based vaccine.
Application : WB
Retallack H et al. PNAS 2016; 113 (50):14408-14413 Zika virus cell tropism in the developing human brain and inhibition by azithromycin.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169