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Zika virus NS3 protein antibody

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

Cat. No. GTX133309

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Zika virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Zika virus NS3 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2756864

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 3 , Zika virus NS3 , ZIKV Non-structural protein 3 , ZIKV NS3

Research Area

DATA IMAGES

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

Mock and infected Vero and BHK-21 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

Non-infected (–) and infected (+) C6/36 cells (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

Non-infected (–) and infected (+) vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133309

GTX133309 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS3 protein antibody (GTX133309).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS3 protein antibody (GTX133309) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

The data was published in the journal BMC Med in 2020.PMID: 32731873

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

The data was published in the PLoS Pathog in 2018. PMID: 30142214

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

The data was published in the 2022 in Viruses. PMID: 35215967

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2017.PMID: 28481898

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

The data was published in the journal Front Microbiol in 2019. PMID: 30984137

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

The data was published in the journal PLoS Pathog in 2017. PMID: 28542603

REFERENCE

REVIEW

Zika virus NS3 protein antibody Cat. No. GTX133309

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Date : Anonymous submitted on 03-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169