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Zika virus NS3 protein antibody

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

Cat No. GTX133309

Reference 17
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Zika virus
Package
100 μl ($359),
25 μl ($169)
See all Zika virus NS3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Zika virus NS3 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Non-structural protein 3 , Zika virus NS3 , ZIKV Non-structural protein 3 , ZIKV NS3

Research Area

DATA IMAGES

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

Mock and infected Vero and BHK-21 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133309

GTX133309 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS3 protein antibody (GTX133309).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS3 protein antibody (GTX133309) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

Non-infected (–) and infected (+) vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

Non-infected (–) and infected (+) C6/36 cells (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

REFERENCE

Application Reference

Guang Song et al. bioRxiv 2020; (Epub) An Integrated Systems Biology Approach Identifies the Proteasome as a Critical Host Machinery for ZIKV and DENV Replication.
Application : WB
Wu Y et al. PLoS Negl Trop Dis 2020; 14 (3) : e0008083 C19orf66 interrupts Zika virus replication by inducing lysosomal degradation of viral NS3.
Application : WB
Zhang X et al. MBio 2019; 10 (5) Zika Virus NS2A-Mediated Virion Assembly.
Application : WB
Li W et al. FASEB J 2019; : fj201900260R Zika virus circumvents host innate immunity by targeting the adaptor proteins MAVS and MITA.
Barrows NJ et al. Sci Rep 2019; 9 (1) : 9711 Dual roles for the ER membrane protein complex in flavivirus infection: viral entry and protein biogenesis.
Application : WB
Show More +
Submit a Reference

REVIEW

Zika virus NS3 protein antibody Cat No. GTX133309

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 03-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Submit a Review
Application Reference
Guang Song et al. bioRxiv 2020; (Epub) An Integrated Systems Biology Approach Identifies the Proteasome as a Critical Host Machinery for ZIKV and DENV Replication.
Application : WB
Wu Y et al. PLoS Negl Trop Dis 2020; 14 (3):e0008083 C19orf66 interrupts Zika virus replication by inducing lysosomal degradation of viral NS3.
Application : WB
Zhang X et al. MBio 2019; 10 (5) Zika Virus NS2A-Mediated Virion Assembly.
Application : WB
Nandan S. Gokhale et al. bioRxiv 2019; (Epub) Altered m6A modification of specific cellular transcripts affects Flaviviridae infection
Application : WB
Li W et al. FASEB J 2019;:fj201900260R Zika virus circumvents host innate immunity by targeting the adaptor proteins MAVS and MITA.
Barrows NJ et al. Sci Rep 2019; 9 (1):9711 Dual roles for the ER membrane protein complex in flavivirus infection: viral entry and protein biogenesis.
Application : WB
Sher AA et al. Front Microbiol 2019; 10:596 Zika Virus Infection Disrupts Astrocytic Proteins Involved in Synapse Control and Axon Guidance.
Application : WB
Taguwa S et al. Cell Rep 2019; 26 (4):906-920.e3 Zika Virus Dependence on Host Hsp70 Provides a Protective Strategy against Infection and Disease.
Application : ICC/IF
Wang K et al. PLoS Pathog 2018; 14 (8):e1007287 *-stimulated trim69 interrupts dengue virus replication by ubiquitinating viral nonstructural protein 3.
Application : WB
Margot Cervantes-Salazar et al. bioRxiv 2018; (Epub) The Nonstructural Proteins 3 And 5 From Flavivirus Modulate Nuclear-Cytoplasmic Transport And Innate Immune Response Targeting Nuclear Proteins.
Application : WB
McFadden MJ et al. Viruses 2018; 10 (2) A Fluorescent Cell-Based System for Imaging Zika Virus Infection in Real-Time.
Application : WB
Chen J et al. Nat Microbiol 2018; 3 (3):302-309 axl promotes zika virus infection in astrocytes by antagonizing type i * signalling.
Application : WB
Boigard H et al. PLoS Negl Trop Dis 2017; 11 (5):e0005608 Zika virus-like particle (VLP) based vaccine.
Li Z et al. Antiviral Res 2017; 150:217-225 Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease.
Application : WB
Brecher M et al. PLoS Pathog 2017; 13 (5):e1006411 A conformational switch high-throughput screening assay and allosteric inhibition of the flavivirus NS2B-NS3 protease.
Application : WB
Boigard H et al. PLoS Negl Trop Dis 2017; (Epub) Zika virus-like particle (VLP) based vaccine.
Application : WB
Retallack H et al. PNAS 2016; 113 (50):14408-14413 Zika virus cell tropism in the developing human brain and inhibition by azithromycin.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 359
$ 169