GeneTex

  • Country / Location Selection

United States (US)

Zika virus NS3 protein antibody

Orthogonal
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Anti-Zika virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133309

Cat. No. GTX133309

Reference 46
9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS3 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Zika virus NS3 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2756864

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 3 , Zika virus NS3 , ZIKV Non-structural protein 3 , ZIKV NS3

Research Area

DATA IMAGES

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

Mock and infected Vero and BHK-21 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

Non-infected (–) and infected (+) C6/36 cells (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309

GTX133309 WB Image

Non-infected (–) and infected (+) vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133309

GTX133309 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS3 protein antibody (GTX133309).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS3 protein antibody (GTX133309) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Pan P et al. Signal Transduct Target Ther 2023; 8 (1) : 194 SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Kao YT et al. Mol Ther Oncolytics 2023; 28 : 104-117 Zika virus cleaves GSDMD to disseminate prognosticable and controllable oncolysis in a human glioblastoma cell model.
Jung HG et al. Emerg Microbes Infect 2022; 11 (1) : 2447-2465 Influence of Zika virus 3'-end sequence and nonstructural protein evolution on the viral replication competence and virulence.
Wang J et al. J Biol Chem 2022; 298 (10) : 102471 A cathelicidin antimicrobial peptide from Hydrophis cyanocinctus inhibits Zika virus infection by downregulating expression of a viral entry factor.
Show More +
Submit a Reference

REVIEW

Zika virus NS3 protein antibody Cat. No. GTX133309

Rating
( Average 5 based on 9 users reviews)
Application
Western Blot(WB)
( Average 5 based on 9 users reviews)
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2022 in Viruses. PMID: 35215967
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 22-Feb-2022
Score :
Application Tested : WB
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the PLoS Pathog in 2018. PMID: 30142214
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Negl Trop Dis in 2017.PMID: 28481898
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal BMC Med in 2020.PMID: 32731873
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017. PMID: 28542603
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2019. PMID: 30984137
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 03-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133309
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Show More +
Submit a Review
Application Reference
Pan P et al. Signal Transduct Target Ther 2023; 8 (1):194 SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Kao YT et al. Mol Ther Oncolytics 2023; 28:104-117 Zika virus cleaves GSDMD to disseminate prognosticable and controllable oncolysis in a human glioblastoma cell model.
Jung HG et al. Emerg Microbes Infect 2022; 11 (1):2447-2465 Influence of Zika virus 3'-end sequence and nonstructural protein evolution on the viral replication competence and virulence.
Wang J et al. J Biol Chem 2022; 298 (10):102471 A cathelicidin antimicrobial peptide from Hydrophis cyanocinctus inhibits Zika virus infection by downregulating expression of a viral entry factor.
Mahamud-Ur Rashid et al. Viruses 2022; 14 (2) Zika Virus Infection of Sertoli Cells Alters Protein Expression Involved in Activated Immune and Antiviral Response Pathways, Carbohydrate Metabolism and Cardiovascular Disease.
Application : WB
Kung YA et al. mBio 2022;:e0271721 Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.
Application : ICC/IF
Patthara Kongsuphol et al. Commun Med (Lond) 2021; 1:46 A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Xi Yu et al. Proc Natl Acad Sci U S A 2021; 118 (42) A mutation-mediated evolutionary adaptation of Zika virus in mosquito and mammalian host.
Application : WB
Weiqiang Chen et al. Nat Microbiol 2021; 6 (4):455-466 Zika virus NS3 protease induces bone morphogenetic protein-dependent brain calcification in human fetuses.
Application : ICC/IF
Jun Shu et al. Emerg Microbes Infect 2021; 10 (1):1609-1625 NS5-independent Ablation of STAT2 by Zika virus to antagonize interferon signalling.
Application : WB
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12):3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Kongsuphol P. et al. Communications Medicine 2021; (Epub) A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Jhan MK et al. Cells 2021; 10 (11) Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.
Application : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
Application : WB
Gambino F Jr et al. Cell Rep 2021; 35 (6):109107 A vaccine inducing solely cytotoxic T lymphocytes fully prevents Zika virus infection and fetal damage.
Application : WB
Li Z et al. Emerg Microbes Infect 2020; 9 (1):2404-2416 Methylene blue is a potent and broad-spectrum inhibitor against Zika virus in vitro and in vivo.
Chen J et al. mBio 2020; 11 (5) Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
Yuan J et al. BMC Med 2020; 18 (1):204 Antibiotic fidaxomicin is an RdRp inhibitor as a potential new therapeutic agent against Zika virus.
Chen Q et al. Nat Commun 2020; 11 (1):2967 Metabolic reprogramming by Zika virus provokes inflammation in human placenta.
Clark AE et al. J Virol 2020; (Epub) Zika virus is transmitted in neural progenitor cells via cell-to-cell spread and infection is inhibited by the autophagy inducer trehalose.
Application : WB
Elong Ngono A et al. Sci Adv 2020; 6 (45) CD8+ T cells mediate protection against Zika virus induced by an NS3-based vaccine.
Application : WB
Li Z et al. ACS Infect Dis 2020; 6 (10):2616-2628 JMX0207, a Niclosamide Derivative with Improved Pharmacokinetics, Suppresses Zika Virus Infection Both In Vitro and In Vivo.
Application : WB
Yuan S et al. Sci Adv 2020; 6 (35):eaba7910 Viruses harness Yxx? motif to interact with host AP2M1 for replication: A vulnerable broad-spectrum antiviral target.
Application : IHC-P
De Jes?s-Gonz?lez LA et al. Viruses 2020; 12 (6) The Nuclear Pore Complex: A Target for NS3 Protease of Dengue and Zika Viruses.
Application : WB
Guang Song et al. bioRxiv 2020; (Epub) An Integrated Systems Biology Approach Identifies the Proteasome as a Critical Host Machinery for ZIKV and DENV Replication.
Application : WB
Wu Y et al. PLoS Negl Trop Dis 2020; 14 (3):e0008083 C19orf66 interrupts Zika virus replication by inducing lysosomal degradation of viral NS3.
Application : WB
Wen F et al. J Biol Chem 2019; 294 (49):18742-18755 Zika virus increases mind bomb 1 levels, causing degradation of pericentriolar material 1 (PCM1) and dispersion of PCM1-containing granules from the centrosome.
Whelan JN et al. J Virol 2019; 93 (16) Zika Virus Production Is Resistant to RNase L Antiviral Activity.
Zhang X et al. MBio 2019; 10 (5) Zika Virus NS2A-Mediated Virion Assembly.
Application : WB
Nandan S. Gokhale et al. bioRxiv 2019; (Epub) Altered m6A modification of specific cellular transcripts affects Flaviviridae infection
Application : WB
Li W et al. FASEB J 2019;:fj201900260R Zika virus circumvents host innate immunity by targeting the adaptor proteins MAVS and MITA.
Barrows NJ et al. Sci Rep 2019; 9 (1):9711 Dual roles for the ER membrane protein complex in flavivirus infection: viral entry and protein biogenesis.
Application : WB
Sher AA et al. Front Microbiol 2019; 10:596 Zika Virus Infection Disrupts Astrocytic Proteins Involved in Synapse Control and Axon Guidance.
Application : WB
Taguwa S et al. Cell Rep 2019; 26 (4):906-920.e3 Zika Virus Dependence on Host Hsp70 Provides a Protective Strategy against Infection and Disease.
Application : ICC/IF
Wang K et al. PLoS Pathog 2018; 14 (8):e1007287 *-stimulated trim69 interrupts dengue virus replication by ubiquitinating viral nonstructural protein 3.
Application : WB
Margot Cervantes-Salazar et al. bioRxiv 2018; (Epub) The Nonstructural Proteins 3 And 5 From Flavivirus Modulate Nuclear-Cytoplasmic Transport And Innate Immune Response Targeting Nuclear Proteins.
Application : WB
McFadden MJ et al. Viruses 2018; 10 (2) A Fluorescent Cell-Based System for Imaging Zika Virus Infection in Real-Time.
Application : WB
Chen J et al. Nat Microbiol 2018; 3 (3):302-309 axl promotes zika virus infection in astrocytes by antagonizing type i * signalling.
Application : WB
Li Z et al. Cell Res 2017; 27 (8):1046-1064 Existing drugs as broad-spectrum and potent inhibitors for Zika virus by targeting NS2B-NS3 interaction.
Boigard H et al. PLoS Negl Trop Dis 2017; 11 (5):e0005608 Zika virus-like particle (VLP) based vaccine.
Li Z et al. Antiviral Res 2017; 150:217-225 Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease.
Application : WB
Brecher M et al. PLoS Pathog 2017; 13 (5):e1006411 A conformational switch high-throughput screening assay and allosteric inhibition of the flavivirus NS2B-NS3 protease.
Application : WB
Boigard H et al. PLoS Negl Trop Dis 2017; (Epub) Zika virus-like particle (VLP) based vaccine.
Application : WB
Retallack H et al. PNAS 2016; 113 (50):14408-14413 Zika virus cell tropism in the developing human brain and inhibition by azithromycin.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169