GeneTex
United States (US)

Zika virus NS3 protein antibody

GTX133309_42508_20160617_WB_Zika_3_18121410_606.jpg
GTX133309_42564_20170831_IFA_18121410_680.jpg
GTX133309_42508_20160617_WB_Zika_18121410_613.jpg
GTX133309_42508_20160617_WB_Zika_2_18121410_910.jpg

Cat No. GTX133309

Reference 9
3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Zika virus
See all Zika virus NS3 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Zika virus NS3 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zika virus NS3 protein antibody , Zika virus NS3 protein antibody

Research Area

DATA IMAGES
GTX133309_42508_20160617_WB_Zika_3_18121410_606.jpg

GTX133309 WB Image

Mock and infected Vero and BHK-21 whole cell extracts (20 µg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

GTX133309_42564_20170831_IFA_18121410_680.jpg

GTX133309 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS3 protein antibody (GTX133309).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS3 protein antibody (GTX133309) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
GTX133309_42508_20160617_WB_Zika_18121410_613.jpg

GTX133309 WB Image

Non-infected (–) and infected (+) vero cells (15 µg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

GTX133309_42508_20160617_WB_Zika_2_18121410_910.jpg

GTX133309 WB Image

Non-infected (–) and infected (+) C6/36 cells (20 µg) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133309) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

REFERENCE

Application Reference

Wang K et al. PLoS Pathog 2018; 14 (8):e1007287 Interferon-stimulated TRIM69 interrupts dengue virus replication by ubiquitinating viral nonstructural protein 3.
Application : WB
Margot Cervantes-Salazar et al. bioRxiv 2018; (Epub ahead of print) The Nonstructural Proteins 3 And 5 From Flavivirus Modulate Nuclear-Cytoplasmic Transport And Innate Immune Response Targeting Nuclear Proteins.
Application : WB
McFadden MJ et al. Viruses 2018; 10 (2) A Fluorescent Cell-Based System for Imaging Zika Virus Infection in Real-Time.
Application : WB
Chen J et al. Nat Microbiol 2018; 3 (3):302-309 AXL promotes Zika virus infection in astrocytes by antagonizing type I interferon signalling.
Application : WB
Boigard H et al. PLoS Negl Trop Dis 2017; 11 (5):e0005608 Zika virus-like particle (VLP) based vaccine.
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Submit a Reference
REVIEW

Zika virus NS3 protein antibody Cat No. GTX133309

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, overnightHr
Date : Anonymous submitted on 03-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, overnightHr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 359
$ 169