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Zika virus NS3 protein antibody

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133320
Anti-Zika virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133320
Anti-Zika virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133320

Cat. No. GTX133320

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS3 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

Zika-infected Vero

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Full length Zika virus NS3 recombinant protein. (Zika virus (strain H/PF/2013))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886926

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 3 , Zika virus NS3 , ZIKV Non-structural protein 3 , ZIKV NS3

Research Area

DATA IMAGES

Anti-Zika virus NS3 protein antibody used in Western Blot (WB). GTX133320

GTX133320 WB Image

Non-infected (–) and infected (+) Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus NS3 protein antibody (GTX133320) diluted at 1:2500.

Anti-Zika virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133320

GTX133320 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus NS3 protein antibody (GTX133320).
Green: Zika virus NS3 protein antibody (GTX133320) diluted at 1:500.

Anti-Zika virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133320

GTX133320 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS3 protein antibody (GTX133320).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS3 protein antibody (GTX133320) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Z Tan et al. PLoS Pathog 2023; 19 (9) : e1011620 LGP2 directly interacts with flavivirus NS5 RNA-dependent RNA polymerase and downregulates its pre-elongation activities
Application : WB
Laura Riva et al. J Virol 2021; 95 (22) : e0099621 The Compound SBI-0090799 Inhibits Zika Virus Infection by Blocking De Novo Formation of the Membranous Replication Compartment.
Application : WB
Shin HJ et al. Microorganisms 2021; 9 (3) Structure-Based Virtual Screening: Identification of a Novel NS2B-NS3 Protease Inhibitor with Potent Antiviral Activity against Zika and Dengue Viruses.
Application : WB
Sarah Goellner et al. STAR Protoc 2020; 1 (3) : 100173 Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.
Application : WB, ICC/IF
Cerikan B et al. Cell Rep 2020; 32 (1) : 107859 A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169