Zika virus NS4A protein antibody

Cat. No. GTX133704

Reference 10

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Zika virus
Package	100 μl ($399), 25 μl ($169)

See all Zika virus NS4A protein products

View product citations for antibody GTX133704 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Zika virus NS4A protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887067

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 4A , Zika virus NS4A , ZIKV Non-structural protein 4A , ZIKV NS4A

Research Area

DATA IMAGES

GTX133704 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS4A protein antibody (GTX133704).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS4A protein antibody (GTX133704) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX133704 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus NS4A protein antibody (GTX133704) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133704 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus NS4A protein antibody (GTX133704) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133704 ICC/IF Image

Immunofluorescent analysis of mock and Zika virus-infected cells using Zika virus NS4A protein antibody (GTX133704).
Sample: Zika virus non-infected and infected cells slide.
Green: Zika virus NS4A protein antibody (GTX133704) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).