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Zika virus NS4B protein antibody [HL1663]

Recombinant antibody
Anti-Zika virus NS4B protein antibody [HL1663] used in Western Blot (WB). GTX637261
Anti-Zika virus NS4B protein antibody [HL1663] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637261
Anti-Zika virus NS4B protein antibody [HL1663] used in Western Blot (WB). GTX637261
Anti-Zika virus NS4B protein antibody [HL1663] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX637261

Cat. No. GTX637261

1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1663

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS4B protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Full length Zika virus NS4B recombinant protein. (strain:"H/PF/2013")

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Zika virus NS4B protein antibody [HL1663] used in Western Blot (WB). GTX637261

GTX637261 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody [HL1663] (GTX637261) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS4B protein antibody [HL1663] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637261

GTX637261 ICC/IF Image

Immunofluorescent analysis of mock and Zika virus-infected cells using Zika virus NS4B protein antibody [HL1663]antibody (GTX637261).
Sample: Zika virus non-infected and infected cells slide.
Green: Zika virus NS4B protein antibody [HL1663]antibody (GTX637261) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Zika virus NS4B protein antibody [HL1663] used in Western Blot (WB). GTX637261

GTX637261 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody [HL1663] (GTX637261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS4B protein antibody [HL1663] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX637261

GTX637261 IHC-P (cell pellet) Image

Zika virus NS4B protein antibody [HL1663] detects Zika virus NS4B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Zika virus NS4B transfected 293T.
Green: Zika virus NS4B stained by Zika virus NS4B protein antibody [HL1663] (GTX637261) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

Zika virus NS4B protein antibody [HL1663] Cat. No. GTX637261

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Zika virus NS4B protein antibody [HL1663] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637261
Date : Anonymous submitted on 15-Feb-2024
Score :
Application Tested : ICC/IF
Sample : VeroE6 cells with/without ZIKV virus infection (MOI 0.1, 24hr)
Blocking : PBS, 2% BSA, 0.5Hr
Fixation : 4 % formal- dehyde
Permeabilization /Antigen retrieval : PBS, 0.5 % Triton X-100, 20 mM glycine
Primary Antibody : 1:1000, °C, Hr
Figure Note :
Orange : Primary antibody
Blue : DAPI
Images were acquired using a Leica SP8 Laser Scanning Confocal Microscope with a 63x oil objective (Leica) and Leica Application Suit X software.
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Package List Price ($)
$ 399
$ 169
Custom Conjugations