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Zika virus NS4B protein antibody

Orthogonal
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311
Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311

Cat. No. GTX133311

Reference 18
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, FACS

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS4B protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus NS4B protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2728825

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 4B , Zika virus NS4B , ZIKV Non-structural protein 4B , ZIKV NS4B

Research Area

DATA IMAGES

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133311) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311

GTX133311 ICC/IF Image

Immunofluorescent analysis of Zika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus NS4B protein antibody (GTX133311).
Green: Zika virus NS4B protein antibody (GTX133311) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311

GTX133311 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS4B antibody (GTX133311).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS4B protein antibody (GTX133311) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133311) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Non-infected (–) and infected (+) vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133311) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Orthogonal

The data was published in the journal Cell Rep in 2019.PMID: 31067454

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Orthogonal

The data was published in the journal Viruses in 2020. PMID: 32138181

Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311

GTX133311 ICC/IF Image

The data was published in the journal Viruses in 2020. PMID: 32138181

REFERENCE

Application Reference

He J et al. Viruses 2023; 15 (6) Zika Virus Induces Degradation of the Numb Protein Required through Embryonic Neurogenesis.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12) : 3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Kolpikova EP et al. Viruses 2020; 12 (3) IRE1α Promotes Zika Virus Infection via XBP1.
Show More +
Submit a Reference

REVIEW

Zika virus NS4B protein antibody Cat. No. GTX133311

Rating
( Average 4.5 based on 4 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Flow cytometry(FACS)
( Average 4 based on 1 users reviews)
Anti-Zika virus NS4B protein antibody used in Flow cytometry (FACS). GTX133311
Date : Anonymous submitted on 26-Oct-2018
Score :
Application Tested : FACS
Sample Species : ZIKV
Sample : Zika Virus infected vero cells
Blocking : PBS + 1% FCS, 4°C, During stainingHr
Fixation : eBioscience? Foxp3 / Transcription Factor Staining Buffer Set
Permeabilization /Antigen retrieval : eBioscience? Foxp3 / Transcription Factor Staining Buffer Set
Primary Antibody : 1:100, 4°C, 1Hr
Figure Note : Red: Mock, Blue: ZIKV infected
Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, 16Hr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Date : Anonymous submitted on 03-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Submit a Review
Application Reference
He J et al. Viruses 2023; 15 (6) Zika Virus Induces Degradation of the Numb Protein Required through Embryonic Neurogenesis.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12):3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Mart?nez LE et al. J Virol 2020; 94 (9) Zika Virus Mucosal Infection Provides Protective Immunity.
Kolpikova EP et al. Viruses 2020; 12 (3) IRE1α Promotes Zika Virus Infection via XBP1.
Jia He et al. Autophagy 2020; (Epub) Zika virus NS2A protein induces the degradation of KPNA2 (karyopherin subunit alpha 2) via chaperone-mediated autophagy.
Application : WB
Roth H et al. Transfusion 2020; 60 (3):561-574 Zika Virus Infection Studies With CD34 + Hematopoietic and Megakaryocyte-Erythroid Progenitors, Red Blood Cells and Platelets.
Application : ICC/IF, FACS
Evans AS et al. J Virol 2020; 94 (9) BPIFB3 Regulates Endoplasmic Reticulum Morphology To Facilitate Flavivirus Replication.
Garcia G Jr et al. Am J Pathol 2020; (Epub) Hippo Signaling Pathway Has a Critical Role in Zika Virus Replication and in the Pathogenesis of Neuroinflammation.
Application : WB
Lin DL et al. Cell Rep 2019; 27 (6):1666-1674.e4 The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection.
Application : WB
Barrows NJ et al. Sci Rep 2019; 9 (1):9711 Dual roles for the ER membrane protein complex in flavivirus infection: viral entry and protein biogenesis.
Application : WB
Cortese M et al. Cell Rep 2019; 27 (9):2579-2592.e6 Reciprocal Effects of Fibroblast Growth Factor Receptor Signaling on Dengue Virus Replication and Virion Production.
Esser-Nobis K et al. J Virol 2019; (Epub) Comparative analysis of African and Asian lineage-derived Zika Virus strains reveals differences in activation of and sensitivity to antiviral innate immunity.
Application : ICC/IF
Wesley Freppel et al. Bio Protoc 2018; 8 (24):e3115 Production, Titration and Imaging of Zika Virus in Mammalian Cells.
Application : ICC/IF
Scaturro P et al. Nature 2018; 561 (7722):253-257 An orthogonal proteomic survey uncovers novel Zika virus host factors.
Application : WB
Azia S Evans et al. bioRxiv 2018; 2018 (Epub) BPIFB3 facilitates flavivirus infection by controlling RETREG1-dependent reticulophagy.
Application : ICC/IF
Martinez L.E. et al. bioRxiv 2017; (Epub) Pathogenesis of Zika Virus Infection via Rectal Route.
Application : IHC-Fr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169