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Zika virus NS4B protein antibody

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311
Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

Cat No. GTX133311

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, FACS

Reactivity

Zika virus
Package
100 μl ($359),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Zika virus NS4B-transfected 293T , Zika virus NS4B-infected C6/36

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus NS4B protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2728825

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Non-structural protein 4B , Zika virus NS4B , ZIKV Non-structural protein 4B , ZIKV NS4B

Research Area

DATA IMAGES

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Non-infected (–) and infected (+) vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133311) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311

GTX133311 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS4B antibody (GTX133311).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS4B protein antibody (GTX133311) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311

GTX133311 ICC/IF Image

Immunofluorescent analysis of Zika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus NS4B protein antibody (GTX133311).
Green: Zika virus NS4B protein antibody (GTX133311) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133311) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133311) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Zika virus NS4B protein antibody Cat No. GTX133311

Rating ( Average 4.7 based on 7 users reviews)
Application
Western Blot(WB) ( Average 4.8 based on 4 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Rep in 2019.PMID: 31067454
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the journal Viruses in 2020.PMID: 32138181
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the journal Viruses in 2020.PMID: 32138181
Date : Anonymous submitted on 26-Oct-2018
Score :
Application Tested : FACS
Sample Species : ZIKV
Sample : Zika Virus infected vero cells
Blocking : PBS + 1% FCS, 4°C, During stainingHr
Fixation : eBioscience? Foxp3 / Transcription Factor Staining Buffer Set
Permeabilization /Antigen retrieval : eBioscience? Foxp3 / Transcription Factor Staining Buffer Set
Primary Antibody : 1:100, 4°C, 1Hr
Figure Note : Red: Mock, Blue: ZIKV infected
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, 16Hr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Date : Anonymous submitted on 03-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf