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Zika virus NS4B protein antibody

Orthogonal
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311
Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311

Cat. No. GTX133311

Reference 17
7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, FACS

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS4B protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus NS4B protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2728825

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Non-structural protein 4B , Zika virus NS4B , ZIKV Non-structural protein 4B , ZIKV NS4B

Research Area

DATA IMAGES

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Non-infected (–) and infected (+) vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133311) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133311) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133311) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311

GTX133311 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS4B antibody (GTX133311).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS4B protein antibody (GTX133311) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311

GTX133311 ICC/IF Image

Immunofluorescent analysis of Zika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus NS4B protein antibody (GTX133311).
Green: Zika virus NS4B protein antibody (GTX133311) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

REFERENCE

Application Reference

Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12) : 3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Mart?nez LE et al. J Virol 2020; 94 (9) Zika Virus Mucosal Infection Provides Protective Immunity.
Kolpikova EP et al. Viruses 2020; 12 (3) IRE1α Promotes Zika Virus Infection via XBP1.
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REVIEW

Zika virus NS4B protein antibody Cat. No. GTX133311

Rating
( Average 4.7 based on 7 users reviews)
Application
Western Blot(WB)
( Average 4.8 based on 4 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
Flow cytometry(FACS)
( Average 4 based on 1 users reviews)
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Rep in 2019.PMID: 31067454
Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the journal Viruses in 2020. PMID: 32138181
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the journal Viruses in 2020. PMID: 32138181
Anti-Zika virus NS4B protein antibody used in Flow cytometry (FACS). GTX133311
Date : Anonymous submitted on 26-Oct-2018
Score :
Application Tested : FACS
Sample Species : ZIKV
Sample : Zika Virus infected vero cells
Blocking : PBS + 1% FCS, 4°C, During stainingHr
Fixation : eBioscience? Foxp3 / Transcription Factor Staining Buffer Set
Permeabilization /Antigen retrieval : eBioscience? Foxp3 / Transcription Factor Staining Buffer Set
Primary Antibody : 1:100, 4°C, 1Hr
Figure Note : Red: Mock, Blue: ZIKV infected
Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, 16Hr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Date : Anonymous submitted on 03-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Show More +
Submit a Review
Application Reference
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12):3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Mart?nez LE et al. J Virol 2020; 94 (9) Zika Virus Mucosal Infection Provides Protective Immunity.
Kolpikova EP et al. Viruses 2020; 12 (3) IRE1α Promotes Zika Virus Infection via XBP1.
Jia He et al. Autophagy 2020; (Epub) Zika virus NS2A protein induces the degradation of KPNA2 (karyopherin subunit alpha 2) via chaperone-mediated autophagy.
Application : WB
Roth H et al. Transfusion 2020; 60 (3):561-574 Zika Virus Infection Studies With CD34 + Hematopoietic and Megakaryocyte-Erythroid Progenitors, Red Blood Cells and Platelets.
Application : ICC/IF, FACS
Evans AS et al. J Virol 2020; 94 (9) BPIFB3 Regulates Endoplasmic Reticulum Morphology To Facilitate Flavivirus Replication.
Garcia G Jr et al. Am J Pathol 2020; (Epub) Hippo Signaling Pathway Has a Critical Role in Zika Virus Replication and in the Pathogenesis of Neuroinflammation.
Application : WB
Lin DL et al. Cell Rep 2019; 27 (6):1666-1674.e4 The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection.
Application : WB
Barrows NJ et al. Sci Rep 2019; 9 (1):9711 Dual roles for the ER membrane protein complex in flavivirus infection: viral entry and protein biogenesis.
Application : WB
Cortese M et al. Cell Rep 2019; 27 (9):2579-2592.e6 Reciprocal Effects of Fibroblast Growth Factor Receptor Signaling on Dengue Virus Replication and Virion Production.
Esser-Nobis K et al. J Virol 2019; (Epub) Comparative analysis of African and Asian lineage-derived Zika Virus strains reveals differences in activation of and sensitivity to antiviral innate immunity.
Application : ICC/IF
Wesley Freppel et al. Bio Protoc 2018; 8 (24):e3115 Production, Titration and Imaging of Zika Virus in Mammalian Cells.
Application : ICC/IF
Scaturro P et al. Nature 2018; 561 (7722):253-257 An orthogonal proteomic survey uncovers novel Zika virus host factors.
Application : WB
Azia S Evans et al. bioRxiv 2018; 2018 (Epub) BPIFB3 facilitates flavivirus infection by controlling RETREG1-dependent reticulophagy.
Application : ICC/IF
Martinez L.E. et al. bioRxiv 2017; (Epub) Pathogenesis of Zika Virus Infection via Rectal Route.
Application : IHC-Fr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169