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Zika virus NS4B protein antibody

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311
Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

Cat No. GTX133311

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr, FACS
Reactivity Zika virus
Package
100 μl ($359),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Zika virus NS4B-transfected 293T , Zika virus NS4B-infected C6/36
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus NS4B protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zika virus NS4B protein antibody , Zika virus NS4B protein antibody

Research Area

DATA IMAGES
Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Non-infected (–) and infected (+) vero cells (15 µg) were separated by gradient gel, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133311) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311

GTX133311 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS4B antibody (GTX133311).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS4B protein antibody (GTX133311) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133311

GTX133311 ICC/IF Image

Immunofluorescent analysis of Zika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus NS4B protein antibody (GTX133311).
Green: Zika virus NS4B protein antibody (GTX133311) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133311) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133311

GTX133311 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133311) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Zika virus NS4B protein antibody Cat No. GTX133311

Rating ( Average 4.5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 26-Oct-2018
Score :
Application Tested : FACS
Sample Species : ZIKV
Sample : Zika Virus infected vero cells
Blocking : PBS + 1% FCS, 4°C, During stainingHr
Fixation : eBioscience? Foxp3 / Transcription Factor Staining Buffer Set
Permeabilization /Antigen retrieval : eBioscience? Foxp3 / Transcription Factor Staining Buffer Set
Primary Antibody : 1:100, 4°C, 1Hr
Figure Note : Red: Mock, Blue: ZIKV infected
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, overnightHr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Date : Anonymous submitted on 03-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, overnightHr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 359
$ 169