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Zika virus NS5 protein antibody [HL2154]

Recombinant antibody
Anti-Zika virus NS5 protein antibody [HL2154] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638132
Anti-Zika virus NS5 protein antibody [HL2154] used in Western Blot (WB). GTX638132

Cat. No. GTX638132

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2154

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS5 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Synthetic peptide of Zika virus NS5 protein. (strain:"H/PF/2013")

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-Zika virus NS5 protein antibody [HL2154] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638132

GTX638132 ICC/IF Image

Immunofluorescent analysis of Zika virus infected cells using Zika virus NS5 protein antibody [HL2154] antibody (GTX638132).
Sample: Zika virus infected cells slide.
Green: Zika virus NS5 protein antibody [HL2154] antibody (GTX638132) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Zika virus NS5 protein antibody [HL2154] used in Western Blot (WB). GTX638132

GTX638132 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Zika virus NS5 protein antibody [HL2154] (GTX638132) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations