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Zika virus NS5 protein antibody

Orthogonal
Anti-Zika virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133312
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312

Cat. No. GTX133312

Reference 24
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS5 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus NS5 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2750559

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Non-structural protein 5 , Zika virus NS5 , ZIKV Non-structural protein 5 , ZIKV NS5

Research Area

DATA IMAGES

Anti-Zika virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133312

GTX133312 ICC/IF Image

Immunofluorescent analysis of Zika virus infected cells using Zika virus NS5 protein antibody (GTX133312).
Sample: Mock and zika virus-infected cells.
Green: Zika virus NS5 protein antibody (GTX133312) diluted at 1:100.

Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312

GTX133312 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Zika virus NS5 protein antibody (GTX133312) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Pan P et al. Signal Transduct Target Ther 2023; 8 (1) : 194 SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.
Wikan N et al. Pharmaceutics 2022; 14 (12) Alpinetin Suppresses Zika Virus-Induced Interleukin-1β Production and Secretion in Human Macrophages.
Dingwen Hu et al. Virulence 2021; 12 (1) : 1795-1807 LAMR1 restricts Zika virus infection by attenuating the envelope protein ubiquitination.
Application : WB
Jun Shu et al. Emerg Microbes Infect 2021; 10 (1) : 1609-1625 NS5-independent Ablation of STAT2 by Zika virus to antagonize interferon signalling.
Application : WB
Yingchong Wang et al. Viruses 2021; 13 (10) USP38 Inhibits Zika Virus Infection by Removing Envelope Protein Ubiquitination.
Application : WB
Show More +
Submit a Reference

REVIEW

Zika virus NS5 protein antibody Cat. No. GTX133312

Rating
( Average 4.8 based on 4 users reviews)
Application
Western Blot(WB)
( Average 4.8 based on 4 users reviews)
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Rep in 2019.PMID: 31067454
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 29895962
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312
Date : Anonymous submitted on 01-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Submit a Review
Application Reference
Pan P et al. Signal Transduct Target Ther 2023; 8 (1):194 SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.
Wikan N et al. Pharmaceutics 2022; 14 (12) Alpinetin Suppresses Zika Virus-Induced Interleukin-1β Production and Secretion in Human Macrophages.
Patthara Kongsuphol et al. Commun Med (Lond) 2021; 1:46 A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Dingwen Hu et al. Virulence 2021; 12 (1):1795-1807 LAMR1 restricts Zika virus infection by attenuating the envelope protein ubiquitination.
Application : WB
Jun Shu et al. Emerg Microbes Infect 2021; 10 (1):1609-1625 NS5-independent Ablation of STAT2 by Zika virus to antagonize interferon signalling.
Application : WB
Yingchong Wang et al. Viruses 2021; 13 (10) USP38 Inhibits Zika Virus Infection by Removing Envelope Protein Ubiquitination.
Application : WB
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12):3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Kongsuphol P. et al. Communications Medicine 2021; (Epub) A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Wang Y. et al. biochemistry 2021; (Epub) USP38 Inhibits Zika Virus Infection by Removing Envelope Protein Ubiquitination.
Mladinich MC et al. mBio 2021; 12 (4):e0196221 Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells.
Application : WB
Chen Y et al. Antiviral Res 2021; 192:105117 Cinnamic acid inhibits Zika virus by inhibiting RdRp activity.
Application : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
Application : WB
Shereen MA et al. PLoS Negl Trop Dis 2021; 15 (4):e0009362 Zika virus dysregulates the expression of astrocytic genes involved in neurodevelopment.
Chen J et al. mBio 2020; 11 (5) Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
Garc?a CC et al. Front Microbiol 2020; 11:1558 Cellular Organelles Reorganization During Zika Virus Infection of Human Cells.
Chen H et al. Virology 2020; 546:88-97 Antiviral activity of lycorine against Zika virus in vivo and in vitro.
Application : WB
Lin DL et al. Cell Rep 2019; 27 (6):1666-1674.e4 The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection.
Application : WB
Lu CY et al. Viruses 2019; 11 (11) The Rescue and Characterization of Recombinant, Microcephaly-Associated Zika Viruses as Single-Round Infectious Particles.
Reactivity : Zika virus
Esser-Nobis K et al. J Virol 2019; (Epub) Comparative analysis of African and Asian lineage-derived Zika Virus strains reveals differences in activation of and sensitivity to antiviral innate immunity.
Application : WB
Lin S et al. Virology 2019; 527:180-187 zika virus ns5 protein antagonizes type i * production via blocking tbk1 activation.
Application : WB
Reactivity : Zika virus
Taguwa S et al. Cell Rep 2019; 26 (4):906-920.e3 Zika Virus Dependence on Host Hsp70 Provides a Protective Strategy against Infection and Disease.
Fink SL et al. Sci Rep 2018; 8 (1):8989 The Antiviral Drug Arbidol Inhibits Zika Virus.
Application : WB
Chen J et al. Nat Microbiol 2018; 3 (3):302-309 axl promotes zika virus infection in astrocytes by antagonizing type i * signalling.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169