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Zika virus NS5 protein antibody

Orthogonal
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312
Anti-Zika virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133312

Cat. No. GTX133312

Reference 28
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS5 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus NS5 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2750559

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 5 , Zika virus NS5 , ZIKV Non-structural protein 5 , ZIKV NS5

Research Area

DATA IMAGES

Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312

GTX133312 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Zika virus NS5 protein antibody (GTX133312) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133312

GTX133312 ICC/IF Image

Immunofluorescent analysis of Zika virus infected cells using Zika virus NS5 protein antibody (GTX133312).
Sample: Mock and zika virus-infected cells.
Green: Zika virus NS5 protein antibody (GTX133312) diluted at 1:100.

REFERENCE

Application Reference

Z Tan et al. PLoS Pathog 2023; 19 (9) : e1011620 LGP2 directly interacts with flavivirus NS5 RNA-dependent RNA polymerase and downregulates its pre-elongation activities
Application : WB, ICC/IF
Joh-Sin Wu et al. Viruses 2023; 15 (8) : 1762 Development of Zika Virus Mini-Replicon Based Single-Round Infectious Particles as Gene Delivery Vehicles
Yujia Zhu et al. Eur J Med Chem 2023; : 115620 Design, synthesis, and biological evaluation of a series of new anthraquinone derivatives as anti-ZIKV agents
Application : WB
Zhu Y et al. Viruses 2023; 15 (5) Repurposing of Doramectin as a New Anti-Zika Virus Agent.
Pan P et al. Signal Transduct Target Ther 2023; 8 (1) : 194 SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.
Show More +
Submit a Reference

REVIEW

Zika virus NS5 protein antibody Cat. No. GTX133312

Rating
( Average 4.8 based on 4 users reviews)
Application
Western Blot(WB)
( Average 4.8 based on 4 users reviews)
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Rep in 2019.PMID: 31067454
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : ZIKV
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 29895962
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312
Date : Anonymous submitted on 01-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Submit a Review
Application Reference
Z Tan et al. PLoS Pathog 2023; 19 (9):e1011620 LGP2 directly interacts with flavivirus NS5 RNA-dependent RNA polymerase and downregulates its pre-elongation activities
Application : WB, ICC/IF
Joh-Sin Wu et al. Viruses 2023; 15 (8):1762 Development of Zika Virus Mini-Replicon Based Single-Round Infectious Particles as Gene Delivery Vehicles
Yujia Zhu et al. Eur J Med Chem 2023;:115620 Design, synthesis, and biological evaluation of a series of new anthraquinone derivatives as anti-ZIKV agents
Application : WB
Zhu Y et al. Viruses 2023; 15 (5) Repurposing of Doramectin as a New Anti-Zika Virus Agent.
Pan P et al. Signal Transduct Target Ther 2023; 8 (1):194 SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.
Wikan N et al. Pharmaceutics 2022; 14 (12) Alpinetin Suppresses Zika Virus-Induced Interleukin-1β Production and Secretion in Human Macrophages.
Patthara Kongsuphol et al. Commun Med (Lond) 2021; 1:46 A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Dingwen Hu et al. Virulence 2021; 12 (1):1795-1807 LAMR1 restricts Zika virus infection by attenuating the envelope protein ubiquitination.
Application : WB
Jun Shu et al. Emerg Microbes Infect 2021; 10 (1):1609-1625 NS5-independent Ablation of STAT2 by Zika virus to antagonize interferon signalling.
Application : WB
Yingchong Wang et al. Viruses 2021; 13 (10) USP38 Inhibits Zika Virus Infection by Removing Envelope Protein Ubiquitination.
Application : WB
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12):3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Kongsuphol P. et al. Communications Medicine 2021; (Epub) A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Wang Y. et al. biochemistry 2021; (Epub) USP38 Inhibits Zika Virus Infection by Removing Envelope Protein Ubiquitination.
Mladinich MC et al. mBio 2021; 12 (4):e0196221 Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells.
Application : WB
Chen Y et al. Antiviral Res 2021; 192:105117 Cinnamic acid inhibits Zika virus by inhibiting RdRp activity.
Application : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
Application : WB
Shereen MA et al. PLoS Negl Trop Dis 2021; 15 (4):e0009362 Zika virus dysregulates the expression of astrocytic genes involved in neurodevelopment.
Chen J et al. mBio 2020; 11 (5) Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
Garc?a CC et al. Front Microbiol 2020; 11:1558 Cellular Organelles Reorganization During Zika Virus Infection of Human Cells.
Chen H et al. Virology 2020; 546:88-97 Antiviral activity of lycorine against Zika virus in vivo and in vitro.
Application : WB
Lin DL et al. Cell Rep 2019; 27 (6):1666-1674.e4 The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection.
Application : WB
Lu CY et al. Viruses 2019; 11 (11) The Rescue and Characterization of Recombinant, Microcephaly-Associated Zika Viruses as Single-Round Infectious Particles.
Reactivity : Zika virus
Esser-Nobis K et al. J Virol 2019; (Epub) Comparative analysis of African and Asian lineage-derived Zika Virus strains reveals differences in activation of and sensitivity to antiviral innate immunity.
Application : WB
Lin S et al. Virology 2019; 527:180-187 zika virus ns5 protein antagonizes type i * production via blocking tbk1 activation.
Application : WB
Reactivity : Zika virus
Taguwa S et al. Cell Rep 2019; 26 (4):906-920.e3 Zika Virus Dependence on Host Hsp70 Provides a Protective Strategy against Infection and Disease.
Fink SL et al. Sci Rep 2018; 8 (1):8989 The Antiviral Drug Arbidol Inhibits Zika Virus.
Application : WB
Chen J et al. Nat Microbiol 2018; 3 (3):302-309 axl promotes zika virus infection in astrocytes by antagonizing type i * signalling.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169