GeneTex
United States (US)

Zika virus NS5 protein antibody

GTX133312_42508_20170908_WB_B_Zika_18121410_147.jpg
GTX133312_42564_20170908_WB_B_Zika_18121410_547.jpg
GTX133312_42508_20170911_IFA_18121410_565.jpg
GTX133312_42508_20160617_WB_Zika_18121410_571.jpg
GTX133312_42508_20160617_WB_Zika_2_18121410_549.jpg

Cat No. GTX133312

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Zika virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

293T , Zika virus NS5-transfected 293T , Zika virus NS5-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus NS5 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zika virus NS5 protein antibody , Zika virus NS5 protein antibody

Research Area

DATA IMAGES
GTX133312_42508_20170908_WB_B_Zika_18121410_147.jpg

GTX133312 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Zika virus NS5 protein antibody (GTX133312) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133312_42564_20170908_WB_B_Zika_18121410_547.jpg

GTX133312 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Zika virus NS5 protein antibody (GTX133312) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133312_42508_20170911_IFA_18121410_565.jpg

GTX133312 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS5 protein antibody (GTX133312).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS5 protein antibody (GTX133312) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX133312_42508_20160617_WB_Zika_18121410_571.jpg

GTX133312 WB Image

Non-infected (–) and infected (+) vero cells (15 µg) were separated by gradient gel, and the membrane was blotted with Zika virus NS5 protein antibody (GTX133312) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

GTX133312_42508_20160617_WB_Zika_2_18121410_549.jpg

GTX133312 WB Image

Non-infected (–) and infected (+) C6/36 cells (20 µg) were separated by gradient gel, and the membrane was blotted with Zika virus NS5 protein antibody (GTX133312) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

REFERENCE
REVIEW

Zika virus NS5 protein antibody Cat No. GTX133312

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, overnightHr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Date : Anonymous submitted on 01-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 359
$ 169