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Zika virus NS5 protein antibody

Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312
Anti-Zika virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133312
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312

Cat. No. GTX133312

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Zika virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus NS5 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2750559

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 5 , Zika virus NS5 , ZIKV Non-structural protein 5 , ZIKV NS5

Research Area

DATA IMAGES

Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312

GTX133312 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Zika virus NS5 protein antibody (GTX133312) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133312

GTX133312 ICC/IF Image

Immunofluorescent analysis of Zika virus infected cells using Zika virus NS5 protein antibody (GTX133312).
Sample: Mock and zika virus-infected cells.
Green: Zika virus NS5 protein antibody (GTX133312) diluted at 1:100.

Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312

GTX133312 WB Image

The data was published in the journal Sci Rep in 2018. PMID: 29895962

Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133312

GTX133312 WB Image

The data was published in the journal Cell Rep in 2019.PMID: 31067454

REFERENCE

REVIEW

Zika virus NS5 protein antibody Cat. No. GTX133312

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Date : Anonymous submitted on 01-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169