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Zika virus prM protein antibody

Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305
Anti-Zika virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133305
Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305

Cat No. GTX133305

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Zika virus
Package
100 μl ($359),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

Zika virus(PRVABC59)-infected Vero , Zika virus prM-infected C6/36
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Zika virus mature matrix protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305

GTX133305 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus prM protein antibody (GTX133305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133305

GTX133305 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus M protein antibody (GTX133305).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus M protein antibody (GTX133305) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305

GTX133305 WB Image

Non-infected (–) and infected (+) Vero cells (15 µg) were separated by gradient gel, and the membrane was blotted with Zika PrM antibody (GTX133305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

REFERENCE
REVIEW

Zika virus prM protein antibody Cat No. GTX133305

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 17-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 359
$ 169