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Zika virus prM protein antibody

Anti-Zika virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133305
Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305
Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305

Cat. No. GTX133305

Reference 21
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus prM protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

Zika virus(PRVABC59)-infected Vero , Zika virus prM-infected C6/36

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Zika virus prM protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886923

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Zika virus Premembrane , Zika virus PrM , ZIKV Premembrane , ZIKV PrM

Research Area

DATA IMAGES

Anti-Zika virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133305

GTX133305 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus prM protein antibody (GTX133305).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus prM protein antibody (GTX133305) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305

GTX133305 WB Image

Non-infected (–) and infected (+) Vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika PrM antibody (GTX133305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305

GTX133305 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus prM protein antibody (GTX133305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Rangel MV et al. J Virol 2022; 96 (2) : e0177421 Structurally Conserved Domains between Flavivirus and Alphavirus Fusion Glycoproteins Contribute to Replication and Infectious-Virion Production.
Zhou S et al. Virol Sin 2022; 37 (5) : 685-694 Rottlerin plays an antiviral role at early and late steps of Zika virus infection.
Thompson D et al. PLoS Negl Trop Dis 2022; 16 (7) : e0010588 Zika virus-like particle vaccine fusion loop mutation increases production yield but fails to protect AG129 mice against Zika virus challenge.
Sara B York et al. mSphere 2021; : e0019221 Zika Virus Hijacks Extracellular Vesicle Tetraspanin Pathways for Cell-to-Cell Transmission.
Application : WB
Show More +
Submit a Reference

REVIEW

Zika virus prM protein antibody Cat. No. GTX133305

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Negl Trop Dis in 2017.PMID: 28481898
Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305
Date : Anonymous submitted on 17-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Submit a Review
Application Reference
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Rangel MV et al. J Virol 2022; 96 (2):e0177421 Structurally Conserved Domains between Flavivirus and Alphavirus Fusion Glycoproteins Contribute to Replication and Infectious-Virion Production.
Zhou S et al. Virol Sin 2022; 37 (5):685-694 Rottlerin plays an antiviral role at early and late steps of Zika virus infection.
Thompson D et al. PLoS Negl Trop Dis 2022; 16 (7):e0010588 Zika virus-like particle vaccine fusion loop mutation increases production yield but fails to protect AG129 mice against Zika virus challenge.
Sara B York et al. mSphere 2021;:e0019221 Zika Virus Hijacks Extracellular Vesicle Tetraspanin Pathways for Cell-to-Cell Transmission.
Application : WB
Xi Yu et al. Proc Natl Acad Sci U S A 2021; 118 (42) A mutation-mediated evolutionary adaptation of Zika virus in mosquito and mammalian host.
Application : WB
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12):3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Chen J et al. mBio 2020; 11 (5) Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
Clark AE et al. J Virol 2020; (Epub) Zika virus is transmitted in neural progenitor cells via cell-to-cell spread and infection is inhibited by the autophagy inducer trehalose.
Application : WB
Xiangling Cui et al. Front Pharmacol. 2020; 11:514313 Identification of Theaflavin-3,3'-Digallate as a Novel Zika Virus Protease Inhibitor.
Application : WB
De Lorenzo G et al. J Virol 2020; Zika virus-like particles bearing covalent dimer of envelope protein protect mice from lethal challenge.
Application : WB
Zhang X et al. MBio 2019; 10 (5) Zika Virus NS2A-Mediated Virion Assembly.
Application : WB
Imran M et al. Viruses 2019; 11 (11) Decanoyl-Arg-Val-Lys-Arg-Chloromethylketone: An Antiviral Compound That Acts against Flaviviruses through the Inhibition of Furin-Mediated prM Cleavage.
Application : WB
Tamhankar M et al. Virol J 2019; 16 (1):99 Directional entry and release of Zika virus from polarized epithelial cells.
Application : WB
Sabino C et al. Viruses 2019; 11 (6) Bafilomycin A1 and U18666A Efficiently Impair ZIKV Infection.
Application : WB
Arumugasaamy N et al. Ann Biomed Eng 2018; (Epub) Biomimetic Placenta-Fetus Model Demonstrating Maternal-Fetal Transmission and Fetal Neural Toxicity of Zika Virus.
Application : ICC/IF
Chen J et al. Nat Microbiol 2018; 3 (3):302-309 axl promotes zika virus infection in astrocytes by antagonizing type i * signalling.
Application : WB
Boigard H et al. PLoS Negl Trop Dis 2017; 11 (5):e0005608 Zika virus-like particle (VLP) based vaccine.
Estoppey D et al. Cell Rep 2017; 19 (3):451-460 The Natural Product Cavinafungin Selectively Interferes with Zika and Dengue Virus Replication by Inhibition of the Host Signal Peptidase.
Application : WB, IP
Boigard H et al. PLoS Negl Trop Dis 2017; (Epub) Zika virus-like particle (VLP) based vaccine.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169