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Zranb1b antibody

Anti-Zranb1b antibody used in Western Blot (WB). GTX129068

Cat No. GTX129068

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Zebrafish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

fifteen zebrafish 24 hpf (hours post fertilization) embryos

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish zranb1b. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zinc finger, RAN-binding domain containing 1b , zgc:154177 , zranb1

Cellular Localization

Cytoplasm , Nucleus

Background

Positive regulator of the Wnt signaling pathway that specifically cleaves 'Lys-63'-linked ubiquitin chains. Acts by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription.

Database

Research Area

DATA IMAGES

Anti-Zranb1b antibody used in Western Blot (WB). GTX129068

GTX129068 WB Image

Zranb1b antibody detects Zranb1b protein by western blot analysis.
A. fifteen zebrafish 24 hpf (hours post fertilization) embryos lysate/extract
7.5 % SDS-PAGE
Zranb1b antibody (GTX129068) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 409
$ 219