GeneTex
United States (US)

Zyxin antibody, Internal

GTX88237_2439_WB_18122102_954.jpg

Cat No. GTX88237

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88237-PEP).

Predict Reactivity

Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QQREKPRVQEKQH, from the internal region of the protein sequence according to NP_003452.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zyxin,Esp-2,Hed-2,Zyx

Cellular Localization

Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.

Background

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88237_2439_WB_18122102_954.jpg

GTX88237 WB Image

WB analysis of peripheral blood mononucleocytes lysate using GTX88237 Zyxin antibody, Internal.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329