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alanyl-tRNA synthetase antibody [N3C2], Internal

Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in Western Blot (WB). GTX112477
Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in Western Blot (WB). GTX112477
Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112477

Cat. No. GTX112477

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all alanyl-tRNA synthetase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Mouse liver

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alanyl-tRNA synthetase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036135

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alanyl-tRNA synthetase , CMT2N , EIEE29

Cellular Localization

Cytoplasm

Background

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in Western Blot (WB). GTX112477

GTX112477 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse liver
5% SDS PAGE
GTX112477 diluted at 1:1000

Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in Western Blot (WB). GTX112477

GTX112477 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
D: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX112477 diluted at 1:1000

Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112477

GTX112477 IHC-P Image

alanyl-tRNA synthetase antibody [N3C2], Internal detects AARS protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
alanyl-tRNA synthetase antibody [N3C2], Internal (GTX112477) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Halpert MM et al. FASEB J 2020; 34 (6) : 8082-8101 MHC class I and II peptide homology regulates the cellular immune response.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169