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alpha 1,2 Mannosidase IA antibody

Anti-alpha 1,2 Mannosidase IA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40044
Anti-alpha 1,2 Mannosidase IA antibody used in IHC (Paraffin sections) (IHC-P). GTX40044
Anti-alpha 1,2 Mannosidase IA antibody used in IHC (Paraffin sections) (IHC-P). GTX40044
Anti-alpha 1,2 Mannosidase IA antibody used in Western Blot (WB). GTX40044
Anti-alpha 1,2 Mannosidase IA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40044
Anti-alpha 1,2 Mannosidase IA antibody used in Western Blot (WB). GTX40044
Anti-alpha 1,2 Mannosidase IA antibody used in IHC (Paraffin sections) (IHC-P). GTX40044
Anti-alpha 1,2 Mannosidase IA antibody used in IHC (Paraffin sections) (IHC-P). GTX40044

Cat. No. GTX40044

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all alpha 1,2 Mannosidase IA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1 μg/ml
ICC/IF 5-10 μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

HepG2, NRK, NIH3T3

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide corresponding to amino acids 138-154 of human α1,2-mannosidase IA with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mannosidase alpha class 1A member 1 , HUMM3 , HUMM9 , MAN9

Background

Alpha 1,2-Mannosidase IA is a Type II transmembrane Golgi-resident enzyme that belongs to class I alpha 1,2- Mannosidases (glycosylhydrolase family 47). Alpha 1,2-Mannosidases play an essential role in the maturation of N-glycans to hybrid and complex oligosaccharides in mammalian cells. They can be classified into three subgroups according to their enzymatic activities. The second subgroup includes mammalian Golgi alpha 1,2-Mannosidases IA, IB, and IC. It has been suggested that Golgi mannosidases from this subgroup may play a role in the ERAD (endoplasmic reticulum-associated degradation) of defective glycoproteins.

Database

Research Area

DATA IMAGES

Anti-alpha 1,2 Mannosidase IA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40044

GTX40044 ICC/IF Image

ICC/IF analysis of NRK cells using GTX40044 alpha 1,2 Mannosidase IA antibody at 10 μg/mL. Cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1).

Anti-alpha 1,2 Mannosidase IA antibody used in IHC (Paraffin sections) (IHC-P). GTX40044

GTX40044 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX40044 alpha 1,2 Mannosidase IA antibody at 0.4 μg/mL.

Anti-alpha 1,2 Mannosidase IA antibody used in IHC (Paraffin sections) (IHC-P). GTX40044

GTX40044 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX40044 alpha 1,2 Mannosidase IA antibody at 0.2 μg/mL.

Anti-alpha 1,2 Mannosidase IA antibody used in Western Blot (WB). GTX40044

GTX40044 WB Image

WB analysis of NIH3T3 cells using GTX40044 alpha 1,2 Mannosidase IA antibody.
Lane 1 : 1 μg/mL antibody
Lane 2 : 1 μg/mL antibody + immunizing peptide

Anti-alpha 1,2 Mannosidase IA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40044

GTX40044 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX40044 alpha 1,2 Mannosidase IA antibody at 10 μg/mL.Cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1).

Anti-alpha 1,2 Mannosidase IA antibody used in Western Blot (WB). GTX40044

GTX40044 WB Image

WB analysis of HepG2 cells using GTX40044 alpha 1,2 Mannosidase IA antibody at 0.5 μg/mL.

Anti-alpha 1,2 Mannosidase IA antibody used in IHC (Paraffin sections) (IHC-P). GTX40044

GTX40044 IHC-P Image

IHC-P analysis of rat pancreas tissue using GTX40044 alpha 1,2 Mannosidase IA antibody at 0.2 μg/mL.

Anti-alpha 1,2 Mannosidase IA antibody used in IHC (Paraffin sections) (IHC-P). GTX40044

GTX40044 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX40044 alpha 1,2 Mannosidase IA antibody at 0.4 μg/mL.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399