APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.5-1 μg/ml |
| 5-10 μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HepG2, NRK, NIH3T3
PROPERTIES
Form
Liquid
Buffer
0.01M PBS, 1% BSA
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
synthetic peptide corresponding to amino acids 138-154 of human α1,2-mannosidase IA with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
mannosidase alpha class 1A member 1 , HUMM3 , HUMM9 , MAN9
Background
Alpha 1,2-Mannosidase IA is a Type II transmembrane Golgi-resident enzyme that belongs to class I alpha 1,2- Mannosidases (glycosylhydrolase family 47). Alpha 1,2-Mannosidases play an essential role in the maturation of N-glycans to hybrid and complex oligosaccharides in mammalian cells. They can be classified into three subgroups according to their enzymatic activities. The second subgroup includes mammalian Golgi alpha 1,2-Mannosidases IA, IB, and IC. It has been suggested that Golgi mannosidases from this subgroup may play a role in the ERAD (endoplasmic reticulum-associated degradation) of defective glycoproteins.
Database
Research Area
DATA IMAGES
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GTX40044 WB Image
WB analysis of NIH3T3 cells using GTX40044 alpha 1,2 Mannosidase IA antibody.
Lane 1 : 1 μg/mL antibody
Lane 2 : 1 μg/mL antibody + immunizing peptide
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GTX40044 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX40044 alpha 1,2 Mannosidase IA antibody at 0.2 μg/mL.
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GTX40044 WB Image
WB analysis of HepG2 cells using GTX40044 alpha 1,2 Mannosidase IA antibody at 0.5 μg/mL.
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GTX40044 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX40044 alpha 1,2 Mannosidase IA antibody at 10 μg/mL.Cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1).
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GTX40044 ICC/IF Image
ICC/IF analysis of NRK cells using GTX40044 alpha 1,2 Mannosidase IA antibody at 10 μg/mL. Cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1).
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GTX40044 IHC-P Image
IHC-P analysis of mouse testis tissue using GTX40044 alpha 1,2 Mannosidase IA antibody at 0.4 μg/mL.
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GTX40044 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX40044 alpha 1,2 Mannosidase IA antibody at 0.4 μg/mL.
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GTX40044 IHC-P Image
IHC-P analysis of rat pancreas tissue using GTX40044 alpha 1,2 Mannosidase IA antibody at 0.2 μg/mL.
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REFERENCE
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REVIEW
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