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alpha 1 Antitrypsin antibody [15H10]

Anti-alpha 1 Antitrypsin antibody [15H10] used in Immunoprecipitation (IP). GTX83665
Anti-alpha 1 Antitrypsin antibody [15H10] used in Western Blot (WB). GTX83665
Anti-alpha 1 Antitrypsin antibody [15H10] used in Western Blot (WB). GTX83665
Anti-alpha 1 Antitrypsin antibody [15H10] used in IHC (Paraffin sections) (IHC-P). GTX83665
Anti-alpha 1 Antitrypsin antibody [15H10] used in IHC (Paraffin sections) (IHC-P). GTX83665

Cat No. GTX83665

Host

Mouse

Clonality

Monoclonal

Clone Name

15H10

Isotype

IgG1

Application

WB, IHC-P, IP

Reactivity

Human, Dog, Monkey
Package
100 μl ($459)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:150
IP 2ug/500ul
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SERPINA1 (NP_000286) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

serpin family A member 1 , A1A , A1AT , AAT , PI , PI1 , PRO2275 , alpha1AT , nNIF

Cellular Localization

Secreted,Endoplasmic reticulum

Background

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

Database

Research Area

DATA IMAGES

Anti-alpha 1 Antitrypsin antibody [15H10] used in Immunoprecipitation (IP). GTX83665

GTX83665 IP Image

IP analysis of DDDDK tagged alpha 1 Antitrypsin overexpressed HEK293T lysate using GTX83665 alpha 1 Antitrypsin antibody [15H10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-alpha 1 Antitrypsin antibody [15H10] used in Western Blot (WB). GTX83665

GTX83665 WB Image

WB analysis of HEK293T cells transfected with alpha 1 Antitrypsin plasmid (Right) or empty vector (Left) for 48 hrs using GTX83665 alpha 1 Antitrypsin antibody [15H10].
Loading : 5 ug per lane

Anti-alpha 1 Antitrypsin antibody [15H10] used in Western Blot (WB). GTX83665

GTX83665 WB Image

WB analysis of various cell lines using GTX83665 alpha 1 Antitrypsin antibody [15H10].
Loading : 35 ug per lane

Anti-alpha 1 Antitrypsin antibody [15H10] used in IHC (Paraffin sections) (IHC-P). GTX83665

GTX83665 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83665 alpha 1 Antitrypsin antibody [15H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-alpha 1 Antitrypsin antibody [15H10] used in IHC (Paraffin sections) (IHC-P). GTX83665

GTX83665 IHC-P Image

IHC-P analysis of human liver tissue using GTX83665 alpha 1 Antitrypsin antibody [15H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

There are currently no references for alpha 1 Antitrypsin antibody [15H10] (GTX83665). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for alpha 1 Antitrypsin antibody [15H10] (GTX83665). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459